• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

清道夫受体-C 作为苏云金芽孢杆菌营养期杀虫蛋白 Vip3Aa 的受体，通过内吞作用介导 Vip3Aa 的内化。

Scavenger receptor-C acts as a receptor for Bacillus thuringiensis vegetative insecticidal protein Vip3Aa and mediates the internalization of Vip3Aa via endocytosis.

机构信息

Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

出版信息

PLoS Pathog. 2018 Oct 4;14(10):e1007347. doi: 10.1371/journal.ppat.1007347. eCollection 2018 Oct.

DOI:10.1371/journal.ppat.1007347
PMID:30286203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6191154/
Abstract

The vegetative insecticidal proteins (Vip), secreted by many Bacillus thuringiensis strains during their vegetative growth stage, are genetically distinct from known insecticidal crystal proteins (ICPs) and represent the second-generation insecticidal toxins. Compared with ICPs, the insecticidal mechanisms of Vip toxins are poorly understood. In particular, there has been no report of a definite receptor of Vip toxins to date. In the present study, we identified the scavenger receptor class C like protein (Sf-SR-C) from the Spodoptera frugiperda (Sf9) cells membrane proteins that bind to the biotin labeled Vip3Aa, via the affinity magnetic bead method coupled with HPLC-MS/MS. We then certified Vip3Aa protoxin could interact with Sf-SR-C in vitro and ex vivo. In addition, downregulation of SR-C expression in Sf9 cells and Spodoptera exigua larvae midgut reduced the toxicity of Vip3Aa to them. Coincidently, heterologous expression of Sf-SR-C in transgenic Drosophila midgut significantly enhanced the virulence of Vip3Aa to the Drosophila larvae. Moreover, the complement control protein domain and MAM domain of Sf-SR-C are involved in the interaction with Vip3Aa protoxin. Furthermore, endocytosis of Vip3Aa mediated by Sf-SR-C correlates with its insecticidal activity. Our results confirmed for the first time that Sf-SR-C acts as a receptor for Vip3Aa protoxin and provides an insight into the mode of action of Vip3Aa that will significantly facilitate the study of its insecticidal mechanism and application.

摘要

植物源杀虫蛋白（Vip）是在某些苏云金芽胞杆菌的营养生长阶段分泌的，它们在遗传上不同于已有的杀虫晶体蛋白（ICPs），代表了第二代杀虫毒素。与 ICPs 相比，Vip 毒素的杀虫机制尚未完全阐明。特别是，到目前为止还没有报道 Vip 毒素的明确受体。在本研究中，我们通过亲和磁珠结合 HPLC-MS/MS 方法，从 Spodoptera frugiperda（ Sf9）细胞膜蛋白中鉴定出与生物素标记的 Vip3Aa 结合的清道夫受体 C 类样蛋白（ Sf-SR-C）。然后我们证明 Vip3Aa 原毒素可以在体外和体内与 Sf-SR-C 相互作用。此外， Sf9 细胞和小菜蛾中肠 SR-C 表达下调降低了 Vip3Aa 对它们的毒性。巧合的是， Sf-SR-C 在转基因果蝇中肠的异源表达显著增强了 Vip3Aa 对果蝇幼虫的毒力。此外， Sf-SR-C 的补体调控蛋白结构域和 MAM 结构域参与与 Vip3Aa 原毒素的相互作用。此外， Sf-SR-C 介导的 Vip3Aa 内吞与它的杀虫活性相关。我们的研究结果首次证实 Sf-SR-C 作为 Vip3Aa 原毒素的受体，并深入了解 Vip3Aa 的作用模式，这将极大地促进其杀虫机制和应用的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/367ae7f40eb9/ppat.1007347.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/8884f545cc40/ppat.1007347.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/685f3e0035fe/ppat.1007347.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/20c6fc7ab7d4/ppat.1007347.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/021ef24c76b4/ppat.1007347.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/361c06f95a3f/ppat.1007347.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/97637291d6a0/ppat.1007347.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/367ae7f40eb9/ppat.1007347.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/8884f545cc40/ppat.1007347.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/685f3e0035fe/ppat.1007347.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/20c6fc7ab7d4/ppat.1007347.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/021ef24c76b4/ppat.1007347.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/361c06f95a3f/ppat.1007347.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/97637291d6a0/ppat.1007347.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b205/6191154/367ae7f40eb9/ppat.1007347.g007.jpg

相似文献

1
Scavenger receptor-C acts as a receptor for Bacillus thuringiensis vegetative insecticidal protein Vip3Aa and mediates the internalization of Vip3Aa via endocytosis.清道夫受体-C 作为苏云金芽孢杆菌营养期杀虫蛋白 Vip3Aa 的受体，通过内吞作用介导 Vip3Aa 的内化。
PLoS Pathog. 2018 Oct 4;14(10):e1007347. doi: 10.1371/journal.ppat.1007347. eCollection 2018 Oct.
2
Susceptibility of Spodoptera frugiperda and S. exigua to Bacillus thuringiensis Vip3Aa insecticidal protein.斜纹夜蛾和甜菜夜蛾对苏云金芽孢杆菌 Vip3Aa 杀虫蛋白的敏感性。
J Invertebr Pathol. 2012 Jul;110(3):334-9. doi: 10.1016/j.jip.2012.03.021. Epub 2012 Mar 23.
3
PHB2 affects the virulence of Vip3Aa to Sf9 cells through internalization and mitochondrial stability.PHB2 通过内化和线粒体稳定性影响 Vip3Aa 对 Sf9 细胞的毒力。
Virulence. 2022 Dec;13(1):684-697. doi: 10.1080/21505594.2022.2064596.
4
Intracellular localization and cytotoxicity of Bacillus thuringiensis Vip3Aa against Spodoptera frugiperda (Sf9) cells.苏云金芽孢杆菌 Vip3Aa 对斜纹夜蛾 (Sf9) 细胞的细胞内定位和细胞毒性。
J Invertebr Pathol. 2020 Mar;171:107340. doi: 10.1016/j.jip.2020.107340. Epub 2020 Feb 7.
5
Vip3Aa induces apoptosis in cultured Spodoptera frugiperda (Sf9) cells.Vip3Aa可诱导培养的草地贪夜蛾（Sf9）细胞凋亡。
Toxicon. 2016 Sep 15;120:49-56. doi: 10.1016/j.toxicon.2016.07.019. Epub 2016 Jul 29.
6
Antagonistic Effect of Truncated Fragments of Vip3Aa on the Larvicidal Activity of its Full-length Protein.截短的 Vip3Aa 片段对其全长蛋白杀虫活性的拮抗作用。
Protein Pept Lett. 2021;28(2):131-139. doi: 10.2174/0929866527666200625205846.
7
Sf-FGFR and Sf-SR-C Are Not the Receptors for Vip3Aa to Exert Insecticidal Toxicity in .Sf-FGFR和Sf-SR-C不是Vip3Aa在……中发挥杀虫毒性的受体。
Insects. 2022 Jun 14;13(6):547. doi: 10.3390/insects13060547.
8
Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa.成纤维细胞生长因子受体，一种新型的 Vip3Aa 杀虫晶体蛋白受体。
Toxins (Basel). 2018 Dec 18;10(12):546. doi: 10.3390/toxins10120546.
9
Functional characterization of Vip3Aa from Bacillus thuringiensis reveals the contributions of specific domains to its insecticidal activity.从苏云金芽孢杆菌中鉴定的 Vip3Aa 功能特性揭示了其特定结构域对其杀虫活性的贡献。
J Biol Chem. 2023 Mar;299(3):103000. doi: 10.1016/j.jbc.2023.103000. Epub 2023 Feb 9.
10
Insecticidal Activity and Histopathological Effects of Vip3Aa Protein from on .来自[具体来源]的Vip3Aa蛋白对[具体对象]的杀虫活性及组织病理学效应 。 （你提供的原文信息不完整，我只能按照现有内容尽量准确翻译，你可补充完整后继续让我翻译。）
J Microbiol Biotechnol. 2016 Oct 28;26(10):1774-1780. doi: 10.4014/jmb.1604.04090.

引用本文的文献

1
Resistance to Vip3Aa: A Growing Threat with Unclear Mechanisms and Management Implications.对Vip3Aa的抗性：一种机制不明且具有管理意义的日益严重的威胁。
Insects. 2025 Aug 7;16(8):820. doi: 10.3390/insects16080820.
2
Potential Mechanisms Underlying the Minimal Impact of Cry1Ab1 Protein on .Cry1Ab1蛋白产生最小影响的潜在机制
Int J Mol Sci. 2025 Mar 24;26(7):2924. doi: 10.3390/ijms26072924.
3
TRPML1 ion channel promotes HepaRG cell differentiation under simulated microgravity conditions.瞬时受体电位阳离子通道M型1（TRPML1）在模拟微重力条件下促进HepaRG细胞分化。

本文引用的文献

1
Changes in gene expression and apoptotic response in Spodoptera exigua larvae exposed to sublethal concentrations of Vip3 insecticidal proteins.小菜蛾幼虫暴露于亚致死浓度的 VIP3 杀虫蛋白后基因表达和凋亡反应的变化。
Sci Rep. 2017 Nov 24;7(1):16245. doi: 10.1038/s41598-017-16406-1.
2
Surge in insect resistance to transgenic crops and prospects for sustainability.昆虫对转基因作物的抗药性激增及其可持续性前景。
Nat Biotechnol. 2017 Oct 11;35(10):926-935. doi: 10.1038/nbt.3974.
3
Transgenic cotton coexpressing Vip3A and Cry1Ac has a broad insecticidal spectrum against lepidopteran pests.
NPJ Microgravity. 2025 Mar 15;11(1):9. doi: 10.1038/s41526-025-00461-4.
4
Insights into the structural changes that trigger receptor binding upon proteolytic activation of Bacillus thuringiensis Vip3Aa insecticidal protein.对苏云金芽孢杆菌Vip3Aa杀虫蛋白蛋白水解激活后触发受体结合的结构变化的见解。
PLoS Pathog. 2024 Dec 5;20(12):e1012765. doi: 10.1371/journal.ppat.1012765. eCollection 2024 Dec.
5
A strategy to enhance the insecticidal potency of Vip3Aa by introducing additional cleavage sites to increase its proteolytic activation efficiency.一种通过引入额外的切割位点来提高Vip3Aa的蛋白水解激活效率，从而增强其杀虫效力的策略。
Eng Microbiol. 2023 Mar 17;3(4):100083. doi: 10.1016/j.engmic.2023.100083. eCollection 2023 Dec.
6
Transcriptomic Analysis of the Response of the Larva Midgut to 2913 Infection.转录组分析幼虫中肠对 2913 感染的反应。
Int J Mol Sci. 2024 Oct 10;25(20):10921. doi: 10.3390/ijms252010921.
7
Scavenger Receptor C1 Mediates Toxicity of Binary Toxin from to Ag55 Cells.清道夫受体 C1 介导二元毒素从 到 Ag55 细胞的毒性。
Toxins (Basel). 2024 Aug 21;16(8):369. doi: 10.3390/toxins16080369.
8
Retrotransposon-mediated disruption of a chitin synthase gene confers insect resistance to Bacillus thuringiensis Vip3Aa toxin.反转录转座子介导的几丁质合成酶基因失活赋予昆虫对苏云金芽孢杆菌 Vip3Aa 毒素的抗性。
PLoS Biol. 2024 Jul 2;22(7):e3002704. doi: 10.1371/journal.pbio.3002704. eCollection 2024 Jul.
9
Binding Analysis of Sf-SR-C MAM Domain and Sf-FGFR Ectodomain to Vip3Aa.Sf-SR-C的MAM结构域和Sf-FGFR胞外结构域与Vip3Aa的结合分析
Insects. 2024 Jun 6;15(6):428. doi: 10.3390/insects15060428.
10
In Vivo and In Vitro Interactions between Exopolysaccharides from HD270 and Vip3Aa11 Protein.HD270 胞外多糖与 Vip3Aa11 蛋白的体内和体外相互作用。
Toxins (Basel). 2024 May 7;16(5):215. doi: 10.3390/toxins16050215.
转 Vip3A 和 Cry1Ac 双价基因棉对鳞翅目害虫具有广谱杀虫活性。
J Invertebr Pathol. 2017 Oct;149:59-65. doi: 10.1016/j.jip.2017.08.001. Epub 2017 Aug 4.
4
A Consensus Definitive Classification of Scavenger Receptors and Their Roles in Health and Disease.清道夫受体的共识性权威分类及其在健康与疾病中的作用
J Immunol. 2017 May 15;198(10):3775-3789. doi: 10.4049/jimmunol.1700373.
5
Vip3Aa induces apoptosis in cultured Spodoptera frugiperda (Sf9) cells.Vip3Aa可诱导培养的草地贪夜蛾（Sf9）细胞凋亡。
Toxicon. 2016 Sep 15;120:49-56. doi: 10.1016/j.toxicon.2016.07.019. Epub 2016 Jul 29.
6
A Small Molecule RAS-Mimetic Disrupts RAS Association with Effector Proteins to Block Signaling.一种小分子RAS模拟物破坏RAS与效应蛋白的结合以阻断信号传导。
Cell. 2016 Apr 21;165(3):643-55. doi: 10.1016/j.cell.2016.03.045.
7
Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria.来自昆虫病原细菌的细菌营养期杀虫蛋白（Vip）
Microbiol Mol Biol Rev. 2016 Mar 2;80(2):329-50. doi: 10.1128/MMBR.00060-15. Print 2016 Jun.
8
Bacillus thuringiensis Crystal Protein Cry6Aa Triggers Caenorhabditis elegans Necrosis Pathway Mediated by Aspartic Protease (ASP-1).苏云金芽孢杆菌晶体蛋白Cry6Aa触发由天冬氨酸蛋白酶（ASP-1）介导的秀丽隐杆线虫坏死途径。
PLoS Pathog. 2016 Jan 21;12(1):e1005389. doi: 10.1371/journal.ppat.1005389. eCollection 2016 Jan.
9
Dual mode of action of Bt proteins: protoxin efficacy against resistant insects.Bt蛋白的双重作用模式：原毒素对抗性昆虫的功效
Sci Rep. 2015 Oct 12;5:15107. doi: 10.1038/srep15107.
10
Scavenger Receptors: Emerging Roles in Cancer Biology and Immunology.清道夫受体：在癌症生物学和免疫学中的新作用。
Adv Cancer Res. 2015;128:309-64. doi: 10.1016/bs.acr.2015.04.004. Epub 2015 Jun 17.