米曲霉产麦角硫因
Ergothioneine production with Aspergillus oryzae.
作者信息
Takusagawa Shun, Satoh Yasuharu, Ohtsu Iwao, Dairi Tohru
机构信息
a Graduate School of Chemical Science and Engineering , Hokkaido University , Sapporo , Hokkaido , Japan.
b Graduate School of Engineering , Hokkaido University , Sapporo, Hokkaido , Japan.
出版信息
Biosci Biotechnol Biochem. 2019 Jan;83(1):181-184. doi: 10.1080/09168451.2018.1527210. Epub 2018 Oct 5.
To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine; l-Cys: l-cysteine; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry.
为建立可靠且实用的麦角硫因（ERG）供应体系，我们利用米曲霉（一种用于食品生产的真菌）进行发酵生产ERG。我们在米曲霉中异源过表达粗糙脉孢菌的egt -1和-2基因，并成功生产出ERG（231.0毫克/千克培养基，比野生型高20倍）。缩写：ERG：麦角硫因；HER：组氨酸三甲铵内盐；Cys - HER：组氨酰半胱氨酸亚砜；SAM：S - 腺苷甲硫氨酸；SAH：S - 腺苷高半胱氨酸；l - His：l - 组氨酸；l - Cys：l - 半胱氨酸；LC - ESI - MS：液相色谱 - 电喷雾电离 - 质谱。
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