Zhang Kenneth Yin, Zhang Taiwei, Wei Huanjie, Wu Qi, Liu Shujuan, Zhao Qiang, Huang Wei
Key Laboratory for Organic Electronics and Information Displays and Jiangsu Key Laboratory for Biosensors , Institute of Advanced Materials (IAM) , Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) , Nanjing University of Posts & Telecommunications , 9 Wenyuan Road , Nanjing 210023 , P. R. China . Email:
Xi'an Institute of Flexible Electronics (XIFE) , Northwestern Polytechnical University (NPU) , 127 West Youyi Road , Xi'an 710072 , P. R. China.
Chem Sci. 2018 Aug 3;9(36):7236-7240. doi: 10.1039/c8sc02984a. eCollection 2018 Sep 28.
Many luminescent probes have been developed for intracellular imaging and sensing. During cellular luminescence sensing, it is difficult to distinguish species generated inside cells from those internalized from extracellular environments since they are chemically the same and lead to the same luminescence response of the probes. Considering that endogenous species usually give more information about the physiological and pathological parameters of the cells while internalized species often reflect the extracellular environmental conditions, we herein reported a series of cyclometalated iridium(iii) complexes as phosphorescent probes that are partially retained in the cell membrane during their cellular uptake. The utilization of the probes for sensing and distinguishing between exogenous and endogenous analytes has been demonstrated using hypoxia and hypochlorite as two examples of target analytes. The endogenous analytes lead to the luminescence response of the intracellular probes while the exogenous analytes are reported by the probes retained in the cell membrane during their internalization.
许多用于细胞内成像和传感的发光探针已被开发出来。在细胞发光传感过程中，很难区分细胞内产生的物质和从细胞外环境内化而来的物质，因为它们在化学性质上是相同的，并且会导致探针产生相同的发光响应。考虑到内源性物质通常能提供更多关于细胞生理和病理参数的信息，而内化物质往往反映细胞外环境条件，我们在此报道了一系列环金属化铱（III）配合物作为磷光探针，它们在细胞摄取过程中部分保留在细胞膜中。以缺氧和次氯酸盐作为目标分析物的两个例子，展示了这些探针用于传感和区分外源性和内源性分析物的应用。内源性分析物会导致细胞内探针的发光响应，而外源性分析物则由内化过程中保留在细胞膜中的探针报告。
