Gene expression analysis of potential morphogen signalling modifying factors in Panarthropoda.

BACKGROUND

Morphogen signalling represents a key mechanism of developmental processes during animal development. Previously, several evolutionary conserved morphogen signalling pathways have been identified, and their players such as the morphogen receptors, morphogen modulating factors (MMFs) and the morphogens themselves have been studied. MMFs are factors that regulate morphogen distribution and activity. The interactions of MMFs with different morphogen signalling pathways such as Wnt signalling, Hedgehog (Hh) signalling and Decapentaplegic (Dpp) signalling are complex because some of the MMFs have been shown to interact with more than one signalling pathway, and depending on genetic context, to have different, biphasic or even opposing function. This complicates the interpretation of expression data and functional data of MMFs and may be one reason why data on MMFs in other arthropods than are scarce or totally lacking.

RESULTS

As a first step to a better understanding of the potential roles of MMFs in arthropod development, we investigate here the embryonic expression patterns of (), (), () and () and in the beetle , the spider , the millipede and the onychophoran . This pioneer study represents the first comprehensive comparative data set of these genes in panarthropods.

CONCLUSIONS

Expression profiles reveal a high degree of diversity, suggesting that MMFs may represent highly evolvable nodes in otherwise conserved gene regulatory networks. Conserved aspects of MMF expression, however, appear to concern function in segmentation and limb development, two of the key topics of evolutionary developmental research.