Jha Chandan K, Mir Rashid, Elfaki Imadeldin, Khullar Naina, Rehman Suriya, Javid Jamsheed, Banu Shaheena, Chahal Sukh Mohinder Singh
Department of Human Genetics Punjabi University, Punjab, India.
Department of Medical Lab Technology, Faculty of Applied Medical Sciences, University of Tabuk, Saudi Arabia.
Endocr Metab Immune Disord Drug Targets. 2019;19(1):67-74. doi: 10.2174/1871530318666181005095724.
Studies have evaluated the association of miRNA-423 C>A genotyping with the susceptibility to various diseases such cancers, atherosclerosis and inflammatory bowel disease but the results were contradictory. However, no studies have reported the association between miRNA-423 rs6505162 C>A polymorphism and susceptibility of coronary artery disease. MicroRNAs regulate expression of multiple genes involved in atherogenesis. Therefore, we investigated the association of microRNA-423C>T gene variations with susceptibility to coronary artery disease.
This study was conducted on 100 coronary artery disease patients and 117 matched healthy controls. The genotyping of the microRNA-423 rs6505162C>A was performed by using Amplification refractory mutation system PCR method (ARMS-PCR).
A significant difference was observed in the genotype distribution among the coronary artery disease cases and sex-matched healthy controls (P=0.048). The frequencies of all three genotypes CC, CA, AA reported in the patient's samples were 55%, 41% and 4% and in the healthy controls samples were 55%, 41% and 4% respectively. Our findings showed that the microRNA-423 C>A variant was associated with an increased risk of coronary artery disease in codominant model (OR = 1.96, 95 % CI, 1.12-3.42; RR 1.35(1.05-1.75, p=0.017) of microRNA-423CA genotype and significant association in dominant model (OR 1.97, 95% CI (1.14-3.39), (CA+AA vs CC) and non-significant association for recessive model (OR=1.42, 95%CI=0.42-4.83, P=0.56, AA vs CC+CA).While, the A allele significantly increased the risk of coronary artery disease (OR =1.56, 95 % CI, 1.03-2.37; p=0.035) compared to C allele. Therefore, it was observed that more than 1.96, 1.97 and 1.56 fold increased risk of developing coronary artery disease.
Our findings indicated that microRNA-423 CA genotype and A allele are associated with an increased susceptibility to Coronary artery disease.
已有研究评估了miRNA - 423 C>A基因分型与多种疾病（如癌症、动脉粥样硬化和炎症性肠病）易感性之间的关联，但结果相互矛盾。然而，尚无研究报道miRNA - 423 rs6505162 C>A多态性与冠状动脉疾病易感性之间的关联。微小RNA可调节参与动脉粥样硬化形成的多个基因的表达。因此，我们研究了微小RNA - 423C>T基因变异与冠状动脉疾病易感性之间的关联。
本研究对100例冠状动脉疾病患者和117例匹配的健康对照进行。采用扩增阻滞突变系统PCR方法（ARMS - PCR）对微小RNA - 423 rs6505162C>A进行基因分型。
在冠状动脉疾病病例和性别匹配的健康对照之间，观察到基因型分布存在显著差异（P = 0.048）。患者样本中报告的所有三种基因型CC、CA、AA的频率分别为55%、41%和4%，健康对照样本中的频率分别为55%、41%和4%。我们的研究结果表明，在共显性模型中，微小RNA - 423 C>A变异与冠状动脉疾病风险增加相关（OR = 1.96，95% CI，1.12 - 3.42；RR 1.35(1.05 - 1.75，p = 0.017)为微小RNA - 423 CA基因型，在显性模型中有显著关联（OR 1.97，95% CI(1.14 - 3.39)，(CA + AA vs CC)，在隐性模型中无显著关联（OR = 1.42，95%CI = 0.42 - 4.83，P = 0.56，AA vs CC + CA）。同时，与C等位基因相比，A等位基因显著增加了冠状动脉疾病的风险（OR = 1.56，95% CI，1.03 - 2.37；p = 0.035）。因此，观察到患冠状动脉疾病的风险增加了1.96、1.97和1.56倍以上。
我们的研究结果表明，微小RNA - 423 CA基因型和A等位基因与冠状动脉疾病易感性增加相关。
