Leal Diana Feio da Veiga Borges, Santana da Silva Mayara Natália, Pastana Lucas Favacho, Fernandes Marianne Rodrigues, de Athayde Aidalucy do Socorro Costa, Fernandes Porchera Débora Christina Ricardo, da Silva Cleonardo Augusto, Modesto Antônio André Conde, De Assumpcão Paulo Pimentel, Dos Santos Sidney Emanuel Batista, Dos Santos Ney Pereira Carneiro
Núcleo de Pesquisa em Oncologia, Universidade Federal do Pará, Belém, Brazil.
Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil.
Front Genet. 2022 Mar 2;13:850058. doi: 10.3389/fgene.2022.850058. eCollection 2022.
Tuberculosis (TB) is a chronic infection caused by (Mtb) with high incidence and mortality. Studies reported that host genetic variants might be associated with the risk of tuberculosis. The aim of this study was to perform an association study between 26 single nucleotide polymorphisms (SNPs) and tuberculosis and evaluate whether these SNPs may confer risk factors to tuberculosis in the Amazon population. There were 52 males and 126 females, with total of 178 healthy controls. Genotyping was performed using TaqMan Open Array Genotyping. Ancestry-informative markers were used to estimate the ancestral proportions of the individuals in the case and control groups. The results indicated that the SNPs rs10035440 (DROSHA), rs7372209 (miR26-a1), rs1834306 (miR100), rs4919510 (miR608), and rs10739971 (pri-let-7a-1) were significantly associated with high risk and rs3746444 (miR499) and rs6505162 (miR423), with low risk of developing tuberculosis in the Amazon population. Our study concluded that seven miRNA polymorphisms were associated with tuberculosis. Our study contributes to a better understanding of TB pathogenesis and may promote the development of new diagnostic tools against infection.
结核病（TB）是由结核分枝杆菌（Mtb）引起的一种慢性感染，发病率和死亡率都很高。研究报告称，宿主基因变异可能与结核病风险相关。本研究的目的是对26个单核苷酸多态性（SNP）与结核病进行关联研究，并评估这些SNP是否可能成为亚马逊人群患结核病的风险因素。研究对象包括52名男性和126名女性，共有178名健康对照者。采用TaqMan Open Array基因分型技术进行基因分型。利用祖先信息标记来估计病例组和对照组个体的祖先比例。结果表明，SNP rs10035440（DROSHA）、rs7372209（miR26-a1）、rs1834306（miR100）、rs4919510（miR608）和rs10739971（pri-let-7a-1）与高风险显著相关，而rs3746444（miR499）和rs6505162（miR423）与亚马逊人群患结核病的低风险相关。我们的研究得出结论，7种微小RNA多态性与结核病有关。我们的研究有助于更好地理解结核病发病机制，并可能促进针对结核感染的新诊断工具的开发。
