微小RNA-224（rs188519172 A>G）基因变异与冠状动脉疾病易感性降低相关：一项病例对照研究。

MicroRNA-224 (rs188519172 A>G) Gene Variability is Associated with a Decreased Susceptibility to Coronary Artery Disease: A Case-Control Study.

作者信息

Mir Rashid, Jha Chandan K, Elfaki Imadeldin, Rehman Suriya, Javid Jamsheed, Khullar Naina, Banu Shaheena, Chahal Sukh Mohinder Singh

机构信息

Department of Medical Lab Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia.

Department of Human Genetics, Punjabi University, Patiala, Punjab, India.

出版信息

Microrna. 2019;8(3):198-205. doi: 10.2174/2211536608666181211153859.

DOI:10.2174/2211536608666181211153859

PMID:30539710

Abstract

AIM

The microRNAs regulate the expression of multiple genes involved in diseases such as cancer, diabetes and cardiovascular disease. In this study, we have investigated the association between the miR-224 gene polymorphism (rs188519172A>G) and susceptibility of coronary artery disease CAD.

METHODOLOGY

Hundred CAD patients and 100-matched healthy control were included. Genotyping of the miR-224 (rs188519172A>G) polymorphism was performed using Amplification refractory mutation system PCR method (ARMS-PCR).

RESULTS

A significant difference was observed in the genotype distribution among CAD patients and healthy controls (P=0.018). The frequencies of all three genotypes GG, GA, AA reported in the patient's samples were 33%, 66% and 01%, and in the healthy controls samples, were 16%, 82% and 2% respectively. A multivariate analysis based on logistic regression was conducted for each group to estimate the association between miR-224 rs188519172 genotypes and risk to coronary artery disease. Results show that the miR-224 (rs188519172 A>G) polymorphism was associated with a decreased risk to CAD in a codominant model, GA genotype vs. GG (OR = 0.39 (95 % CI, 0.19-0.76), RR 0.58 (0.38-0.90, P=0.006). In the dominant model, (GA+AA vs. GG), there was also a significant association with the OR=0.38 (95 % CI (0.19-0.76), RR 0.58 (0.38-0.89), and P=0.006. Whereas, in the recessive model, (GG+GA vs. AA), there was no significant association of CAD with OR=0.49 (95% CI (0.044-5.54), RR 0.74 (0.33-1.68), and P=0.48.

CONCLUSION

Our findings indicated that miR-224 (rs188519172) GA genotype is associated with a decreased susceptibility to CAD.

摘要

目的

微小RNA调控多种与癌症、糖尿病和心血管疾病等疾病相关的基因表达。在本研究中，我们调查了miR - 224基因多态性（rs188519172A>G）与冠状动脉疾病（CAD）易感性之间的关联。

方法

纳入100例CAD患者和100例匹配的健康对照。采用扩增阻滞突变系统PCR方法（ARMS - PCR）对miR - 224（rs188519172A>G）多态性进行基因分型。

结果

CAD患者和健康对照的基因型分布存在显著差异（P = 0.018）。患者样本中报告的所有三种基因型GG、GA、AA的频率分别为33%、66%和1%，而在健康对照样本中分别为16%、82%和2%。对每组进行基于逻辑回归的多变量分析，以估计miR - 224 rs188519172基因型与冠状动脉疾病风险之间的关联。结果显示，在共显性模型中，miR - 224（rs188519172 A>G）多态性与CAD风险降低相关，GA基因型与GG基因型相比（比值比=0.39（95%置信区间，0.19 - 0.76），相对风险0.58（0.38 - 0.90），P = 0.006）。在显性模型中，（GA + AA与GG相比），也存在显著关联，比值比=0.38（95%置信区间（0.19 - 0.76），相对风险0.58（0.38 - 0.89），P = 0.006）。然而，在隐性模型中，（GG + GA与AA相比），CAD与比值比=0.49（95%置信区间（0.044 - 5.54），相对风险0.74（0.33 - 1.68），P = 0.48）无显著关联。