Department of Plant Pathology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, 100193 Beijing, P. R. China.
Plant Science college, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, P. R. China.
FEMS Microbiol Lett. 2018 Nov 1;365(22). doi: 10.1093/femsle/fny242.
Bipolaris sorokiniana is a soil-borne fungal pathogen causing common root rot in wheat, and is difficult to control through chemical and agricultural means. Chaetomium spp. have been documented as potential biological control agents for plant diseases. In this study, seven Chaetomium strains were screened for their abilities to control B. sorokiniana on agar plates. Strain 22-10 significantly inhibited the growth of B. sorokiniana on potato dextrose agar plates, up to 66.7%. Its filtrate of liquid culture also inhibited the mycelial growth of B. sorokiniana, indicating that strain 22-10 produced secondary metabolites against B. sorokiniana. The incidence and disease indexes of common root rot significantly decreased in wheat after treatment with the crude extract of strain 22-10. Six active compounds were purified from crude extract of this fungus culture against B. sorokiniana. Chaetoviridin A showed the highest efficiency to inhibit the growth of B. sorokiniana. Strain 22-10 was identified as Chaetomium globosum based on phylogenetic analysis of the rDNA internal transcribed spacer region and microscopic characteristics. The high control efficiency of strain 22-10 of C. globosum against B. sorokiniana suggested it has potential to be a biocontrol agent for B. sorokiniana.
旋孢腔菌是一种土传真菌病原体，可导致小麦常见的根腐病，难以通过化学和农业手段进行控制。管毛霉属真菌已被记录为植物病害的潜在生物防治剂。在这项研究中，筛选了 7 株管毛霉属菌株，以评估它们在琼脂平板上控制旋孢腔菌的能力。菌株 22-10 显著抑制了旋孢腔菌在马铃薯葡萄糖琼脂平板上的生长，抑制率高达 66.7%。其液体培养滤液也抑制了旋孢腔菌的菌丝生长，表明菌株 22-10 产生了针对旋孢腔菌的次生代谢物。用菌株 22-10 的粗提物处理后，小麦的常见根腐病发病率和病情指数显著降低。从该真菌培养物的粗提物中分离出 6 种对旋孢腔菌有活性的化合物。Chaetoviridin A 对抑制旋孢腔菌的生长效率最高。根据 rDNA 内转录间隔区和微观特征的系统发育分析，菌株 22-10 被鉴定为管毛霉属真菌。菌株 22-10 对旋孢腔菌的高效控制效率表明，它具有作为旋孢腔菌生物防治剂的潜力。
