Shandong Agricultural University, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, No. 61 Daizong Street, Taian City, Shandong Province, 271018, China.
Poult Sci. 2019 Feb 1;98(2):664-678. doi: 10.3382/ps/pey415.
The gastrointestinal tract is the site for the uptake of nutrients from the external environment. We hypothesized that the antioxidant system in the intestinal tract has a vital protective role from the oxidative damage induced by oxidants in foods. The aim of this study was to investigate the development of the antioxidant system in the intestine of chickens. The activity and gene expression of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and the content of the non-enzymatic substance glutathione (GSH) were measured in the duodenum, jejunum, and ileum of chickens at 1, 3, 7, 11, 14, 21, 35, and 42 d of age. The results showed that the small intestinal tract had relatively higher SOD activity and GSH concentration and lower CAT and GSH-Px activities, compared with those of other visceral organs. CAT and GSH-Px activities and GSH concentration showed a decreasing trend with age, whereas SOD activity was not significantly influenced by age. The gene expression of SOD1, SOD2, and GSH-Px7 showed a dramatic decrease from 3 d of age. The results indicated that SOD and GSH were highly expressed in the first week of age after hatching. To conclude, the results suggest that SOD and GSH play a vital protective role in the small intestine after hatching, which contributes to rapid development of the intestine.
胃肠道是从外部环境中吸收营养物质的部位。我们假设，肠道中的抗氧化系统对于食物中的氧化剂所诱导的氧化损伤具有重要的保护作用。本研究旨在探讨鸡肠道抗氧化系统的发育情况。在 1、3、7、11、14、21、35 和 42 日龄时，测量了鸡十二指肠、空肠和回肠中抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性和基因表达以及非酶物质谷胱甘肽（GSH）的含量。结果表明，与其他内脏器官相比，鸡的小肠具有相对较高的 SOD 活性和 GSH 浓度，以及较低的 CAT 和 GSH-Px 活性。CAT 和 GSH-Px 活性和 GSH 浓度随日龄呈下降趋势，而 SOD 活性不受日龄影响。SOD1、SOD2 和 GSH-Px7 的基因表达从 3 日龄开始急剧下降。结果表明，SOD 和 GSH 在孵化后第一周高度表达。总之，这些结果表明，SOD 和 GSH 在孵化后对小肠具有重要的保护作用，有助于肠道的快速发育。
