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转染SV40 DNA - gpt重组体后人结肠黏膜上皮细胞的表型变化及基因表达

Phenotypic changes and gene expression in human colon mucosal epithelial cells upon transfection of a SV40 DNA-gpt recombinant.

作者信息

Moyer M P, Aust J B

出版信息

In Vitro Cell Dev Biol. 1987 Feb;23(2):141-6. doi: 10.1007/BF02623595.

DOI:10.1007/BF02623595
PMID:3029012
Abstract

Phenotypic changes (increased longevity, decreased growth factor requirements, altered cell surface features, growth in semisolid agarose, and SV40 T antigen expression) suggesting in vitro transformation were displayed by human normal colon mucosal epithelial cells transfected with pSV3gpt, a pBR322 recombinant containing the SV40 "early" T antigen coding region and the dominant selectable marker bacterial gene, xanthine-guanine phosphoribosyltransferase. In contrast, control cultures which received neither DNA nor the recombinant pSV2gpt (which is identical to pSV3gpt but lacks the SV40 T antigen region) were not phenotypically altered.

摘要

用pSV3gpt转染的人正常结肠黏膜上皮细胞呈现出提示体外转化的表型变化（寿命延长、生长因子需求降低、细胞表面特征改变、在半固体琼脂糖中生长以及SV40 T抗原表达）。pSV3gpt是一种pBR322重组体，包含SV40“早期”T抗原编码区和显性选择标记细菌基因黄嘌呤 - 鸟嘌呤磷酸核糖转移酶。相比之下，既未接受DNA也未接受重组体pSV2gpt（与pSV3gpt相同但缺乏SV40 T抗原区）的对照培养物没有发生表型改变。

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引用本文的文献

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本文引用的文献

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AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.用于多瘤病毒转化细胞选择性测定的琼脂悬浮培养
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Tissue culture of human epithelial cells from benign colonic tumors.良性结肠肿瘤来源的人上皮细胞的组织培养。
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Disrupted communication between late-stage premalignant human colon epithelial cells by 12-O tetradecanoylphorbol-13-acetate.12-O-十四酰佛波醇-13-乙酸酯破坏晚期癌前人类结肠上皮细胞之间的通讯。
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Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.筛选表达编码黄嘌呤 - 鸟嘌呤磷酸核糖转移酶的大肠杆菌基因的动物细胞。
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Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation.人类结肠黏蛋白随细胞分化和恶性转化而发生的改变。
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Colon epithelium. IV. Human colon carcinogenesis. Changes in human colon mucosa adjacent to and remote from carcinomas of the colon.结肠上皮。IV. 人类结肠癌发生。结肠肿瘤旁及远离肿瘤处的人类结肠黏膜变化。
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12-O-Tetradecanoylphorbol-13-acetate stimulation of DNA synthesis in cultured preneoplastic familial polyposis colonic epithelial cells but not in normal colonic epithelial cells.12 - 十四酰佛波醇 - 13 - 乙酸酯刺激培养的癌前家族性息肉病结肠上皮细胞中的DNA合成，但不刺激正常结肠上皮细胞中的DNA合成。
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Actin cytoskeletal organization loss in the benign-to-malignant tumor transition in cultured human colonic epithelial cells.培养的人结肠上皮细胞从良性肿瘤向恶性肿瘤转变过程中肌动蛋白细胞骨架组织的丧失
Cancer Res. 1984 Jul;44(7):3040-50.