Universidad Autónoma de Nayarit, Secretaría de Investigación y Posgrado, Laboratorio de Inmunotoxicología, Boulevard Tepic-Xalisco s/n, Cd. de la Cultura Amado Nervo, C.P. 63000, Tepic, Nayarit, Mexico.
Universidad Autónoma de Nayarit, Secretaría de Investigación y Posgrado, Laboratorio de Inmunotoxicología, Boulevard Tepic-Xalisco s/n, Cd. de la Cultura Amado Nervo, C.P. 63000, Tepic, Nayarit, Mexico; Centro Nayarita de Innovación y Transferencia de Tecnología A.C., Laboratorio Nacional para la Investigación en Inocuidad Alimentaria-Unidad Nayarit, Calle Tres s/n, Cd Industrial, Tepic, Nayarit, Mexico.
Fish Shellfish Immunol. 2019 Jan;84:189-195. doi: 10.1016/j.fsi.2018.10.002. Epub 2018 Oct 4.
Organophosphorus pesticides (OPs) are broad-spectrum insecticides. One of the commonly used OPs is diazinon (DZN). The aim of this study was to evaluate the immunotoxic effect of DZN on phagocytic parameters of blood leukocytes using the teleost fish Oreochromis niloticus as a study model. For this purpose, fish were exposed in vivo to 0.97, 1.95 and 3.97 mg/L of DZN for 6 and 24 h. Our results indicated that phagocytic active cells decreased in fish exposed in vivo to 0.97 and 1.95 mg/L of DZN for 6 and 24 h. Regarding ROS production, HO and O levels were higher on fish exposed to 1.95 mg/L for 6 and 24 h, while HO production increased at 0.97 mg/L for 24 h. From this we can conclude that phagocytic parameters are sensitive to assess the effect of acute intoxication with organophosphorus pesticides on Nile tilapia.
有机磷农药（OPs）是一种广谱杀虫剂。其中一种常用的有机磷农药是敌敌畏（DZN）。本研究旨在利用尼罗罗非鱼作为研究模型，评估 DZN 对血液白细胞吞噬参数的免疫毒性作用。为此，将鱼类暴露于 0.97、1.95 和 3.97 mg/L 的 DZN 中 6 和 24 h。我们的结果表明，暴露于 0.97 和 1.95 mg/L 的 DZN 中 6 和 24 h 的鱼的吞噬活性细胞减少。关于 ROS 产生，HO 和 O 水平在暴露于 1.95 mg/L 的鱼中升高 6 和 24 h，而在 24 h 时 HO 产量增加。由此可以得出结论，吞噬参数敏感，可用于评估急性有机磷农药中毒对尼罗罗非鱼的影响。
