Zeng Bo, Lai Zhiwen, Sun Lijin, Zhang Zhongbao, Yang Jianhua, Li Zaixin, Lin Jie, Zhang Zhi
Department of Pharmaceutical Engineering, Sichuan University of Science & Engineering, 180 Xueyuan Street, Zigong, 643000, Sichuan, China.
Department of Pharmaceutical Engineering, Sichuan University of Science & Engineering, 180 Xueyuan Street, Zigong, 643000, Sichuan, China; Department of Reproductive Medicine Center, Zigong Maternal and Child Health Care Hospital, 180 Longjin Street, Zigong, 643000, Sichuan, China.
Res Microbiol. 2019 Jan-Feb;170(1):43-52. doi: 10.1016/j.resmic.2018.09.002. Epub 2018 Oct 4.
Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder that affects 9-21% of reproductive-aged women. Affected women frequently display obesity, insulin resistance, and inflammation. Altered gut microbial community has been reported in PCOS and obese PCOS patients. However, the profile of the gut microbial community in insulin resistant PCOS (IR-PCOS) patients still remains unknown. In this study, next-generation sequencing based on the 16S rRNA gene was used to compare the gut microbial composition of women with IR-PCOS (n = 9, PCOS with insulin resistance), NIR-PCOS (n = 8, PCOS alone) and healthy controls (n = 8, HC). We assessed that the composition of the gut microbial communities in NIR-PCOS and IR-PCOS patients were significantly altered. The family Bacteroidaceae was prolific in the NIR-PCOS group and reached its highest level in the IR-PCOS group, while the Prevotellaceae dramatically decreased in PCOS patients, especially in the IR-PCOS group. Subsequent correlation analysis revealed that the increased clinical parameter levels, including insulin resistance, sex-hormones and inflammation, were positively associated with the abundance of Bacteroidaceae, but negatively associated with that of Prevotellaceae. In addition, IR-PCOS patients also displayed a significant difference in their amounts of Ruminococcaceae and Lachnospiraceae when compared to the NIR-PCOS group. Moreover, the functional prediction from PICRUSt revealed that 73 pathways are significantly changed in the gut microbial communities of PCOS patients. Specifically, 21 metabolism-associated pathways, including the steroid hormone biosynthesis and lipopolysaccharide biosynthesis pathways, are obviously changed in IR-PCOS when compared to NIR-PCOS and HC groups. Taking this into consideration, our present study suggests that the dysbiosis of gut microbial communities occurred most notably in IR-PCOS patients, and the difference in gut dysbiosis profile between the IR-PCOS and NIR-PCOS should be considered in clinical treatment for PCOS patients and future drugs development.
多囊卵巢综合征（PCOS）是一种复杂的内分泌和代谢紊乱疾病，影响9%至21%的育龄妇女。受影响的女性经常表现出肥胖、胰岛素抵抗和炎症。在PCOS和肥胖的PCOS患者中，肠道微生物群落已被报道发生改变。然而，胰岛素抵抗型PCOS（IR-PCOS）患者的肠道微生物群落特征仍然未知。在本研究中，基于16S rRNA基因的下一代测序被用于比较IR-PCOS患者（n = 9，患有胰岛素抵抗的PCOS）、非胰岛素抵抗型PCOS（NIR-PCOS，n = 8，单纯PCOS）和健康对照（n = 8，HC）女性的肠道微生物组成。我们评估发现，NIR-PCOS和IR-PCOS患者的肠道微生物群落组成发生了显著改变。拟杆菌科在NIR-PCOS组中数量众多，在IR-PCOS组中达到最高水平，而普雷沃氏菌科在PCOS患者中显著减少，尤其是在IR-PCOS组。随后的相关性分析表明，包括胰岛素抵抗、性激素和炎症在内的临床参数水平升高与拟杆菌科的丰度呈正相关，但与普雷沃氏菌科的丰度呈负相关。此外，与NIR-PCOS组相比，IR-PCOS患者的瘤胃球菌科和毛螺菌科数量也存在显著差异。此外，PICRUSt的功能预测显示，PCOS患者肠道微生物群落中有73条途径发生了显著变化。具体而言，与NIR-PCOS和HC组相比，IR-PCOS组中包括类固醇激素生物合成和脂多糖生物合成途径在内的21条代谢相关途径明显改变。考虑到这一点，我们目前的研究表明，肠道微生物群落失调在IR-PCOS患者中最为明显，在PCOS患者的临床治疗和未来药物开发中应考虑IR-PCOS和NIR-PCOS之间肠道失调特征的差异。
