Department of Assisted Reproduction, Xiangya Hospital, Central South University, 410008, Changsha, People's Republic of China.
J Ovarian Res. 2021 Mar 27;14(1):50. doi: 10.1186/s13048-021-00799-9.
Limited studies have reported the relationship between intestinal flora dysbiosis and clinical characteristics in polycystic ovary syndrome (PCOS). However, the structure and characteristics of gut microbiota in PCOS have not been fully elucidated.
To analyze the composition of the Intestinal flora population in normal-weight women with PCOS and insulin resistance(IR) compared to PCOS alone and healthy women.
A total of 14 PCOS patients with insulin resistant(PCOS-IR) and 12 PCOS alone (PCOS-NIR), and 10 age- and body mass index-matched healthy control women (HC). BMI: 18.5-23.9 kg/m. The bacterial 16 S rDNA V3-V4 fragment was amplified and sequenced. Then, the sequencing data were analyzed for species annotation, community diversity, and inter-group differences, to explore gut microbial characteristics of the subjects and their correlation with clinical parameters.
No significant difference in diversity was observed between PCOA and sample cluster analysis among the three groups (Beta-diversity) and Alpha-diversity. The relative abundance of Rothia, Ruminococcus, and Enterococcus was significantly higher in the PCOS-IR group than in the other two groups (P < 0.05), while that of Prevotella was dramatically decreased (P < 0.05). The abundance of Enterococcus was positively correlated with waist circumference, hip circumference, diastolic blood pressure, and insulin resistance index. Meanwhile, Rothia abundance is positively associated with waist circumference and free fatty acids.
The gut microbial composition of PCOS patients with insulin resistance is different from that of PCOS alone and healthy women. The difference is correlated with the clinical characteristics of PCOS, with regards to insulin resistance, abdominal obesity, free fatty acids, and other indicators. PCOS-IR patients have an increased abundance of Enterococcus which potentially the intestinal environment of the host by enriching the metabolic pathways related to insulin resistance, causing the occurrence and development of PCOS.
有限的研究报告了肠道菌群失调与多囊卵巢综合征（PCOS）临床特征之间的关系。然而，PCOS 中肠道微生物群的结构和特征尚未完全阐明。
分析与单纯 PCOS 相比，伴有胰岛素抵抗（IR）的正常体重 PCOS 妇女肠道菌群的组成。
共纳入 14 例胰岛素抵抗的 PCOS 患者（PCOS-IR）、12 例单纯 PCOS 患者（PCOS-NIR）和 10 例年龄和体重指数匹配的健康对照妇女（HC）。BMI：18.5-23.9kg/m。扩增和测序细菌 16S rDNA V3-V4 片段。然后，对测序数据进行物种注释、群落多样性和组间差异分析，以探讨研究对象的肠道微生物特征及其与临床参数的相关性。
三组间（β多样性）和 Alpha 多样性无明显差异。与其他两组相比，PCOS-IR 组中 Rothia、Ruminococcus 和 Enterococcus 的相对丰度显著升高（P<0.05），而 Prevotella 的相对丰度显著降低（P<0.05）。Enterococcus 的丰度与腰围、臀围、舒张压和胰岛素抵抗指数呈正相关。同时，Rothia 的丰度与腰围和游离脂肪酸呈正相关。
伴胰岛素抵抗的 PCOS 患者的肠道微生物组成与单纯 PCOS 患者和健康女性不同。这种差异与 PCOS 患者的临床特征有关，包括胰岛素抵抗、腹型肥胖、游离脂肪酸等指标。PCOS-IR 患者的 Enterococcus 丰度增加，可能通过丰富与胰岛素抵抗相关的代谢途径，使宿主肠道环境发生变化，导致 PCOS 的发生和发展。
