LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast.

The cation-dependent galactose-specific flocculation activity of the null mutant of , the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that disruption of , another flocculation associated regulatory kinase encoding gene, also resulted in cation-dependent galactose-specific flocculation. Deletion of increased the flocculation phenotype of the null mutant and its overexpression reversed the flocculation of cells caused by deletion. Transcript levels of were also decreased by deletion. Cumulatively, these results indicate that Lkh1 is one of the negative regulators acting upstream of Prk1, regulating non-sexual flocculation in fission yeast.