Deguchi H, Kitaura Y, Hayashi T, Kotaka M, Morita H, Kawamura K
Jpn Circ J. 1986 Dec;50(12):1268-74. doi: 10.1253/jcj.50.1268.
This light- and electron-microscopic study using monoclonal antibodies provides an immunohistochemical demonstration of lymphocytes in situ in the myocardium in murine coxsackie B3 virus myocarditis. On the 7th and 9th days after virus inoculation, we observed many necrotic cardiocyte foci infiltrated with numerous inflammatory cells including macrophages and T lymphocytes. There were only a few Lyt 1-bearing lymphocytes in this phase of inoculation. On the 14th day there were many Lyt 1-bearing cells among the T cells in the foci. By immuno-electron microscopy, some Lyt 1-bearing T lymphocytes were seen in close contact with macrophages and/or other lymphocytes. These Lyt 1-bearing lymphocytes appeared to represent helper T cells, although Lyt 2.3+ lymphocytes were not examined. It is well known that activation of helper T cells leads to stimulation of B cells and cytotoxic T cells. It is assumed, therefore, that Lyt 1-bearing T lymphocytes that infiltrated into the myocardial foci play an important role in immune responsiveness at this stage of viral myocarditis.
这项使用单克隆抗体的光镜和电镜研究，对小鼠柯萨奇B3病毒心肌炎心肌中淋巴细胞进行了原位免疫组化显示。在病毒接种后的第7天和第9天，我们观察到许多坏死心肌细胞灶，有大量包括巨噬细胞和T淋巴细胞在内的炎性细胞浸润。在此接种阶段，仅有少数携带Lyt 1的淋巴细胞。在第14天，病灶中的T细胞中有许多携带Lyt 1的细胞。通过免疫电镜观察，可见一些携带Lyt 1的T淋巴细胞与巨噬细胞和/或其他淋巴细胞紧密接触。尽管未检测Lyt 2.3+淋巴细胞，但这些携带Lyt 1的淋巴细胞似乎代表辅助性T细胞。众所周知，辅助性T细胞的激活会导致B细胞和细胞毒性T细胞的刺激。因此可以推测，浸润到心肌病灶中的携带Lyt 1的T淋巴细胞在病毒性心肌炎的这个阶段的免疫反应中起重要作用。
