Venkateswarulu N, Shameer S, Bramhachari P V, Basha S K Thaslim, Nagaraju C, Vijaya T
Department of Botany, Sri Venkateswara University, Tirupati, 517502, A.P, India.
Nanotechnology Laboratory, IFT, RARS, ANGRAU, Tirupati, 517502, A.P, India.
Biotechnol Rep (Amst). 2018 Sep 28;20:e00282. doi: 10.1016/j.btre.2018.e00282. eCollection 2018 Dec.
The rationale of the present study was to isolate and identify endophytic fungi from endemic medicinal plants in Eastern Ghats and screened for antimicrobial potential of isolated fungal crude extracts. A total of 329 endophytic strains were isolated from 600 infected leaves and stem cuttings of endemic plants. The diversity and species richness was analyzed statistically and found to be higher in leaf segments than in stem segments. From isolated fungal strains, was identified using molecular identification methods and selected as the most potent plumbagin-producing endophytic strain. Further the isolation and structural characterization of endophytic fungal plumbagin (5-hydroxyl-2-methylnaptalene-1,4-dione) was purified and confirmed through spectroscopy analysis. The molecular weight was determined as 188 in positive mode by ESI-MS, which confirmed to be plumbagin which potentially inhibited all tested pathogens, therefore the endophytic fungal plumbagin from the current study possesses important biological activities against pathogens.
本研究的目的是从东高止山脉的特有药用植物中分离和鉴定内生真菌，并筛选分离出的真菌粗提物的抗菌潜力。从600份特有植物的感染叶片和茎段中总共分离出329株内生菌株。对其多样性和物种丰富度进行了统计分析，发现叶片部分的多样性和物种丰富度高于茎段。通过分子鉴定方法从分离出的真菌菌株中鉴定出 ，并将其选为最有效的产白花丹素内生菌株。此外，通过光谱分析对内生真菌白花丹素（5-羟基-2-甲基萘-1,4-二酮）进行了分离和结构表征，并进行了纯化和确认。通过电喷雾电离质谱法（ESI-MS）在正模式下测定其分子量为188，证实为白花丹素，它可能抑制所有测试的病原体，因此本研究中的内生真菌白花丹素具有针对病原体的重要生物活性。
