Ragavendran Chinnasamy, Govindaraj Annadurai, Kamaraj Chinnaperumal, Natarajan Devarajan, Malafaia Guilherme, Alrefaei Abdulwahed Fahad, Almutairi Mikhlid H
Department of Cariology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, 600 077 Tamil Nadu India.
Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem, 636 011 Tamil Nadu India.
3 Biotech. 2024 Oct;14(10):226. doi: 10.1007/s13205-024-04061-z. Epub 2024 Sep 10.
Endophytic fungal molecules have the potential to be a cost-effective chemical source for developing eco-friendly disease-controlling pharmaceuticals that target mosquito-borne illnesses. The primary aims of the study were to identify the fungus larvicidal ability against , and The ethyl acetate extract demonstrated lethal concentrations that kill 50% of exposed larvae (LC) and 90% of exposed larvae (LC) for the 1st to 4th instar larvae of (LC = 54.821, 66.525, 68.250, and 73.614; LC = 104.56, 138.205, 150.415, and 159.466 μg/mL), (LC = 64.981, 36.505, 42.230, and 36.514; LC = 180.46, 157.105, 140.318, and 153.366 μg/ mL), and (LC = 74.890, 33.607, 52.173, and 26.974; LC = 202.56, 162.205, 130.518, and 163.286 μg/mL). Mycelium metabolites were evaluated for their pupicidal activity towards (LC = 80.669, LC = 119.904), (LC = 70.569, LC = 109.840), and (LC = 73.269, LC = 110.590 μg/mL). The highest larvicidal activity was recorded at 300 µg/mL, with 100% mortality against first and second-instar larvae of . Metabolite exposure to larvae exhibited several abnormal behavioral changes. The exposure to metabolite, key esterases such as acetylcholinesterase, α-and-β-carboxylesterase, and acid and alkaline phosphatase activity significantly decreased compared to control larvae. The outcomes of the histology analysis revealed that the mycelium metabolites-treated targeted larvae had a disorganized abdominal mid and hindgut epithelial cells. The is first-hand information on study of ethyl-acetate-derived metabolites from tested against larvae and pupae of and . Bio-indicator toxicity findings demonstrate that displayed no mortality.
The online version contains supplementary material available at 10.1007/s13205-024-04061-z.
内生真菌分子有可能成为一种具有成本效益的化学来源，用于开发针对蚊媒疾病的环保型疾病控制药物。该研究的主要目的是确定真菌对[具体蚊虫种类1]、[具体蚊虫种类2]和[具体蚊虫种类3]的杀幼虫能力。乙酸乙酯提取物对[具体蚊虫种类1] 1至4龄幼虫的致死浓度分别为杀死50%暴露幼虫的浓度（LC50）和杀死90%暴露幼虫的浓度（LC90）（LC50 = 54.821、66.525、68.250和73.614；LC90 = 104.56、138.205、150.415和159.466μg/mL），对[具体蚊虫种类2]（LC50 = 64.981、36.505、42.230和36.514；LC90 = 180.46、157.105、140.318和153.366μg/mL），以及对[具体蚊虫种类3]（LC50 = 74.890、33.607、52.173和26.974；LC90 = 202.56、162.205、130.518和163.286μg/mL）。对菌丝体代谢产物针对[具体蚊虫种类1]（LC50 = 80.669，LC90 = 119.904）、[具体蚊虫种类2]（LC50 = 70.569，LC90 = 109.840）和[具体蚊虫种类3]（LC50 = 73.269，LC90 = 110.590μg/mL）的杀蛹活性进行了评估。在300μg/mL时记录到最高杀幼虫活性，对[具体蚊虫种类1]的一龄和二龄幼虫死亡率达100%。代谢产物暴露于幼虫表现出几种异常行为变化。与对照幼虫相比，暴露于[具体真菌种类]代谢产物后，关键酯酶如乙酰胆碱酯酶、α-和β-羧酸酯酶以及酸性和碱性磷酸酶活性显著降低。组织学分析结果显示，经菌丝体代谢产物处理的目标幼虫腹部中肠和后肠上皮细胞紊乱。这是关于测试[具体真菌种类]乙酸乙酯衍生代谢产物对[具体蚊虫种类1]和[具体蚊虫种类2]幼虫和蛹的研究的第一手信息。生物指示毒性研究结果表明[具体对照生物]未出现死亡情况。
在线版本包含可在10.1007/s13205-024-04061-z获取的补充材料。
