Dian Ziqin, Wang Binghui, Fan Mao, Dong Shuwei, Feng Yue, Zhang A-Mei, Liu Li, Niu Hua, Li Yuanyue, Xia Xueshan
Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan 650500, PR China.
Department of Clinical Laboratory, The First People's Hospital of Yunnan Province, Yunnan 650032, PR China.
J Gen Virol. 2017 Jun;98(6):1163-1168. doi: 10.1099/jgv.0.000807. Epub 2017 Jun 14.
G9P[8] rotavirus A (RVA) has been identified as the predominant genotype circulating in Yunnan, China. To elucidate the molecular characteristics of its genetic composition at the whole-genome level, the genomes of 12 strains isolated from paediatric patients with diarrhoea were fully sequenced and characterized. Eleven of the 12 strains were genotyped as G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, which is closely related to the Wa-like genotype 1 constellation. In contrast, one strain was genotyped as G9-P[8]-I1-R1-C1-M1-A1-N2-T1-E1-H1, with the NSP2 gene characterized as a DS-1 like genotype. Bayesian phylogenetic analysis indicated that G9 strains had emerged in 1932 with an estimated average evolutionary rate of 1.63×10-3 substitutions/site/year. Considering the high prevalence and fast evolutionary rate of G9P[8] rotaviruses, our results suggest that G9P[8] RVA should be strictly monitored in China.
G9P[8] 轮状病毒A（RVA）已被确定为在中国云南流行的主要基因型。为了阐明其全基因组水平遗传组成的分子特征，对从腹泻小儿患者中分离出的12株病毒的基因组进行了全序列测定和特征分析。12株病毒中有11株被基因分型为G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1，这与Wa样基因型1星座密切相关。相比之下，有一株被基因分型为G9-P[8]-I1-R1-C1-M1-A1-N2-T1-E1-H1，其NSP2基因被表征为DS-1样基因型。贝叶斯系统发育分析表明，G9毒株于1932年出现，估计平均进化速率为1.63×10-3个替换/位点/年。考虑到G9P[8] 轮状病毒的高流行率和快速进化速率，我们的结果表明在中国应严格监测G9P[8] RVA。
