Liu Jia-Ji, Zhang Xuan, Wu Xiao-Hou
Department of Urology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402160, China.
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400015, China.
Mol Ther Oncolytics. 2018 Aug 16;11:14-19. doi: 10.1016/j.omto.2018.08.001. eCollection 2018 Dec 21.
This study aimed to evaluate the effects of miR-93 on the growth and invasiveness of prostate cancer (PC) cells (PCCs). Real-time PCR was carried out to detect the expression of miR-93 in the PC tissues and cell lines. The adjacent normal tissues served as controls. For experiments, methyl thiazolyl tetrazolium, clone formation, tumor cell invasion assays, and western blot analysis (WBA) were performed to confirm the variations in the proliferation and invasiveness of PCCs, prior and subsequent to transfection with an miR-93 antisense oligonucleotide (ASO), which blocks miR-93 binding to its target. Furthermore, the effect of miR-93 on the proliferation of PCCs was examined. Finally, the expression levels of the target genes of miR-93 were determined by WBA. miR-93 expression was higher in PC tissues than in the adjacent normal tissues, and a reduction in the miR-93 level remarkably inhibited the proliferation and invasiveness of PCCs. Moreover, miR-93 enhanced the expression of its target genes , , and . The results of this study suggest that miR-93 may promote the proliferation and invasion of PCCs by upregulating its target genes , , and .
本研究旨在评估miR-93对前列腺癌细胞（PCCs）生长和侵袭能力的影响。采用实时定量聚合酶链反应（Real-time PCR）检测miR-93在前列腺癌组织及细胞系中的表达情况，以相邻正常组织作为对照。实验中，通过甲基噻唑基四氮唑蓝（MTT）比色法、克隆形成实验、肿瘤细胞侵袭实验以及蛋白质免疫印迹分析（WBA），来确认在转染可阻断miR-93与其靶标结合的miR-93反义寡核苷酸（ASO）前后，PCCs增殖和侵袭能力的变化。此外，还检测了miR-93对PCCs增殖的影响。最后，通过蛋白质免疫印迹分析确定miR-93靶基因的表达水平。结果显示，miR-93在前列腺癌组织中的表达高于相邻正常组织，降低miR-93水平可显著抑制PCCs的增殖和侵袭能力。此外，miR-93增强了其靶基因[靶基因1]、[靶基因2]和[靶基因3]的表达。本研究结果表明，miR-93可能通过上调其靶基因[靶基因1]、[靶基因2]和[靶基因3]来促进PCCs的增殖和侵袭。
