Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India.
Methods Mol Biol. 2020;2125:157-166. doi: 10.1007/7651_2018_194.
Dental pulp stem cells (DPSCs) are adult multipotent stem cells of neuroectodermal origin; they provide an encouraging perspective in the domain of nerve tissue engineering. DPSCs could be transplanted in biodegradable electrospun neuro-supportive scaffold (optimized in various 3D geometries like coating on the surface of titanium implant, hollow/solid tubes, etc.) for enhanced in vivo recovery of peripheral nerves. Herein, we describe the fabrication of uniform bead-free nanofibrous scaffold which supports DPSCs, proliferation, and their subsequent neural differentiation and thus could be utilized for enhanced regeneration of peripheral nervous system.
牙髓干细胞（DPSCs）是神经外胚层来源的成体多能干细胞；它们在神经组织工程领域提供了令人鼓舞的前景。DPSCs 可以被移植到可生物降解的电纺神经支持支架中（通过各种 3D 几何形状进行优化，如钛植入物表面的涂层、空心/实心管等），以增强周围神经的体内恢复。在这里，我们描述了制造均匀的无珠纳米纤维支架的方法，该支架支持 DPSCs 的增殖及其随后的神经分化，因此可用于增强周围神经系统的再生。
