Embaló Bubacar, Parize Hian Nivaldo, Rivero Elena Riet Correa
Postgraduate Program in Dentistry, Health Sciences Center, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Undergraduate Course in Dentistry, Health Sciences Center, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Microsc Res Tech. 2018 Nov;81(11):1241-1245. doi: 10.1002/jemt.23128. Epub 2018 Oct 8.
The purpose of this study was to evaluate the metabolism and epithelial cell proliferation of odontogenic keratocyst (OKC), dentigerous cyst (DC), and unicystic ameloblastoma (UA) by quantifying the nucleolar organizing regions (AgNORs) and Ki-67 protein immunoexpression. Forty-eight cases (16 OKC, 16 DC, and 16 UA) were evaluated retrospectively. The metabolism and epithelial cell proliferation was measured by the Ki-67 positive cell percentage index and by the mean AgNOR count in each group. The Ki-67 and AgNOR counts were significantly higher in OKC comparing to the DC and UA (p < .001). Ki-67 positive cells were observed higher in suprabasal cell layers of OKC with uniform distribution, a few of them were predominantly observed in basal cell layer in DC and UA. The AgNOR count was significantly higher in the OKC basal cell layers and observed throughout the lining epithelium of DC and UA. Ki-67 and AgNOR reinforced the aggressive character of OKC, presenting high metabolism and cellular proliferation compared to DC and UA, possibly due to its more aggressive clinical behavior and high recurrence rate. RESEARCH HIGHLIGHTS: We evidence higher metabolism and epithelial cell proliferation in OKC when compared to UA and DC, supporting its aggressive aspect and its high rate of recurrence. OKC had intense and predominant labeling of Ki-67 on the suprabasal layer unlike UA and DC.
本研究的目的是通过定量核仁组织区（AgNORs）和Ki-67蛋白免疫表达来评估牙源性角化囊肿（OKC）、含牙囊肿（DC）和单囊性成釉细胞瘤（UA）的代谢及上皮细胞增殖情况。对48例病例（16例OKC、16例DC和16例UA）进行回顾性评估。通过Ki-67阳性细胞百分比指数和每组的平均AgNOR计数来测量代谢及上皮细胞增殖情况。与DC和UA相比，OKC中的Ki-67和AgNOR计数显著更高（p < 0.001）。在OKC的基底上层观察到较高的Ki-67阳性细胞，分布均匀，而在DC和UA中，少数Ki-67阳性细胞主要出现在基底细胞层。OKC基底细胞层的AgNOR计数显著更高，而在DC和UA的整个衬里上皮中均观察到AgNOR。Ki-67和AgNOR强化了OKC的侵袭性特征，与DC和UA相比，其具有更高的代谢和细胞增殖，这可能归因于其更具侵袭性的临床行为和高复发率。研究亮点：与UA和DC相比，我们证实OKC具有更高的代谢和上皮细胞增殖，支持其侵袭性方面及其高复发率。与UA和DC不同，OKC在基底上层有强烈且主要的Ki-67标记。
