Sobue G, Yasuda T, Mitsuma T, Pleasure D
Neurosci Lett. 1986 Dec 23;72(3):253-7. doi: 10.1016/0304-3940(86)90522-7.
By using indirect immunofluorescence, galactocerebroside (galC) was detected on the surface of Schwann cells cultured from unmyelinated fibers of 10- to 12-day-old rat cervical sympathetic nerve trunk. By day 4 in vitro, galC-positive cells disappeared from the culture. When the 4-day cultures were treated with 1 mM 8-bromo cyclic AMP or dibutyryl cyclic AMP, galC reappeared in 72 h. The proportion of Schwann cells expressing galC was dependent on the concentration of cyclic AMP derivatives used.
通过间接免疫荧光法，在从10至12日龄大鼠颈交感神经干无髓纤维培养的雪旺细胞表面检测到半乳糖脑苷脂（galC）。在体外培养的第4天，galC阳性细胞从培养物中消失。当对培养4天的细胞用1 mM 8-溴环磷酸腺苷或二丁酰环磷酸腺苷处理时，galC在72小时后重新出现。表达galC的雪旺细胞比例取决于所用环磷酸腺苷衍生物的浓度。
