Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
J Dent. 2018 Dec;79:68-76. doi: 10.1016/j.jdent.2018.10.002. Epub 2018 Oct 5.
To investigate the anti-biofilm efficacy of root canal irrigants in canal spaces, isthmi and dentinal tubules of root canals ex vivo.
Fifty-one single-rooted premolars, each containing an isthmus, were instrumented, autoclaved and inoculated with Enterococcus faecalis for 4 weeks. One specimen was sectioned for bacteria-specific staining to confirm the presence of biofilms using light microscopiy. The remaining specimens were randomly divided to five groups: (1) 0.9% NaCl, (2) SilverSol/HO, (3) HYBENX, (4) QMix 2 in1, (5) 6% NaOCl. Bacterial sampling was performed before (S1) and after (S2) canal irrigation. Diluted bacteria suspension was cultured for 48 h for counting the colony forming units (CFU). Percentages of dead bacteria and biofilm thickness were evaluated by confocal laser scanning microscopy (CLSM). Metabolic activity, lactic acid and polysaccharide synthesis of E. faecalis derived from S2 samples were analysed.
The percentages of dead bacteria were significantly affected by the factor "irrigant" (p < 0.001) and the factor "location" (p = 0.017). The percentages of dead bacteria in the isthmi and canals were both in the ordor: NaCl < SilverSol/HO < HYBENX < QMix 2 in1 < NaOCl (p < 0.05). Only 6% NaOCl disrupted biofilms and significantly reduced their thickness. The CFU, metabolic activity, polysaccharide and lactic acid production of E. faecalis were all reduced by the disinfecting solutions.
SilverSol/HO and HYBENX were less adept than QMix 2 in1 at killing biofilm bacteria in root canals. None of these antibacterial irrigants were effective, compared with 6% NaOCl, in disrupting biofilms.
There is advantage in using HYBENX or QMix 2 in1 to kill intratubular bacteria biofilms because of their capability in removing the inorganic component of the smear layer. SilverSol/HO requires extra time to eradicate intratubular biofilms upon removal of the organic and inorganic components of the smear layer by other root canal irrigants.
研究根管冲洗液在根管内、峡部和牙本质小管内的抗生物膜效果。
51 颗单根前磨牙，每颗都有一个峡部，经过器械预备、高压灭菌和粪肠球菌接种 4 周。一个标本用于细菌特异性染色，使用光学显微镜确认生物膜的存在。其余标本随机分为五组：（1）0.9%NaCl，（2）SilverSol/HO，（3）HYBENX，（4）QMix 2 in1，（5）6%NaOCl。在根管冲洗前（S1）和后（S2）进行细菌取样。稀释后的细菌悬液培养 48 小时，计算菌落形成单位（CFU）。通过共聚焦激光扫描显微镜（CLSM）评估死菌百分比和生物膜厚度。分析 S2 样本中粪肠球菌的代谢活性、乳酸和多糖合成。
死菌百分比受“冲洗液”因素（p<0.001）和“位置”因素（p=0.017）显著影响。峡部和根管内的死菌百分比顺序为：NaCl<QMix 2 in1<HYBENX<SilverSol/HO<NaOCl（p<0.05）。只有 6%NaOCl 能破坏生物膜并显著降低其厚度。消毒溶液均降低了粪肠球菌的 CFU、代谢活性、多糖和乳酸的产生。
SilverSol/HO 和 HYBENX 不如 QMix 2 in1 擅长杀死根管内的生物膜细菌。与 6%NaOCl 相比，这些抗菌冲洗液都不能有效地破坏生物膜。
由于 HYBENX 或 QMix 2 in1 具有去除玷污层无机成分的能力，因此在杀死管内细菌生物膜方面具有优势。SilverSol/HO 需要额外的时间来消除其他根管冲洗液去除玷污层的有机和无机成分后管内的生物膜。
