Nasim Iffat, Shamly Marimuthu, Jaju KrishnaKanth, Vishnupriya Veeraraghavan, Jabin Zohra
Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, India.
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077, India.
Bioinformation. 2022 May 31;18(5):450-454. doi: 10.6026/97320630018450. eCollection 2022.
The most common intracanal medication is calcium hydroxide. Its efficacy can be affected by a number of factors, including pH, serum proteins, collagen, and dentin. It's also ineffective against E. faecalis and fungus, lacks an anti-inflammatory component, and has mixed reviews when it comes to pain relief. Natural alternatives to synthetic intracanal medication are being researched at the moment. We evaluated the antioxidant and anti-inflammatory activity of green synthesized silver nanoparticle based intracanal medicaments. Silver nanoparticles integrated into calcium hydroxide and graphene oxide nanoparticles were the experimental groups and Calcium hydroxide served as the control. Antioxidant activity was determined using the DPPH and Nitric oxide assays, while anti-inflammatory activity was determined using the protein denaturation and Xanthine Oxidase Inhibition assays. Both experimental groups had higher antioxidant activity than the control group based on DPPH and Nitric oxide assays. Calcium hydroxide combined with silver nanoparticles demonstrated improved anti-inflammatory efficacy in a protein denaturation and Xanthine oxidase inhibition assay. Within the constraints of an in vitro study, it can be concluded that intracanal medicaments containing silver nanoparticles can be employed efficiently during root canal preparation. In comparison to standard calcium hydroxide-based intracanal medicaments, it has effective antioxidant and anti-inflammatory effects.
最常见的根管内用药是氢氧化钙。其疗效会受到多种因素的影响，包括pH值、血清蛋白、胶原蛋白和牙本质。它对粪肠球菌和真菌也无效，缺乏抗炎成分，在缓解疼痛方面口碑不一。目前正在研究合成根管内用药的天然替代品。我们评估了基于绿色合成银纳米颗粒的根管内药物的抗氧化和抗炎活性。整合到氢氧化钙和氧化石墨烯纳米颗粒中的银纳米颗粒为实验组，氢氧化钙作为对照组。使用DPPH和一氧化氮测定法测定抗氧化活性，同时使用蛋白质变性和黄嘌呤氧化酶抑制测定法测定抗炎活性。基于DPPH和一氧化氮测定法，两个实验组的抗氧化活性均高于对照组。在蛋白质变性和黄嘌呤氧化酶抑制试验中，氢氧化钙与银纳米颗粒结合显示出更好的抗炎效果。在体外研究的限制范围内，可以得出结论，含银纳米颗粒的根管内药物在根管预备过程中可以有效使用。与标准的氢氧化钙基根管内药物相比，它具有有效的抗氧化和抗炎作用。
