酿酒酵母基因PHO2的序列编码一种具有异常氨基酸组成的调节蛋白。
The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual aminoacid composition.
作者信息
Sengstag C, Hinnen A
出版信息
Nucleic Acids Res. 1987 Jan 12;15(1):233-46. doi: 10.1093/nar/15.1.233.
Abstract
A new centromere vector for the construction of a Saccharomyces cerevisiae gene library, allowing direct selection for DNA insert, will be described. From that library the gene for the regulatory protein PHO2 involved in PHO5 induction has been cloned by complementation of a pho2 mutation. The complementing activity was shown to be located on a 3.6 kb HindIII fragment. This fragment was used to evict the genomic copy and with appropriate genetic crosses we proved, that the cloned gene is PHO2. The DNA sequence of PHO2 was determined. Analysis of the sequence data uncovered striking homology regions with PHO4, another protein necessary for the induction of PHO5. The relevance of the observed homology will be discussed.
摘要
将介绍一种用于构建酿酒酵母基因文库的新型着丝粒载体，该载体可直接筛选DNA插入片段。利用该文库，通过对pho2突变的互补作用，克隆了参与PHO5诱导的调节蛋白PHO2的基因。互补活性定位于一个3.6 kb的HindIII片段上。该片段用于取代基因组拷贝，通过适当的遗传杂交，我们证明克隆的基因就是PHO2。测定了PHO2的DNA序列。对序列数据的分析揭示了与PHO4（另一种诱导PHO5所必需的蛋白质）的显著同源区域。将讨论所观察到的同源性的相关性。
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