通过寡核苷酸介导诱变构建的改良型阳性选择质粒载体。
An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis.
作者信息
Nilsson B, Uhlén M, Josephson S, Gatenbeck S, Philipson L
出版信息
Nucleic Acids Res. 1983 Nov 25;11(22):8019-30. doi: 10.1093/nar/11.22.8019.
Abstract
An Escherichia coli plasmid vector, pUN121, has been constructed which allows for positive selection of transformants harboring DNA inserts. The positive selection of transformants harboring DNA inserts. The vector is based on plasmid pTR262 (Roberts et al. Gene, 12, (1980), 123-127) in which the tetracycline resistance gene is under transcriptional control of the repressor protein coded by the phage lambda cI gene. This plasmid has been rearranged, using in vitro recombinant techniques including oligonucleotide mediated mutagenesis to yield a smaller plasmid (4.4 kb) with unique cloning sites for EcoRI, XmaI and SmaI in addition to the unique HindIII and BclI sites. The plasmid has a functional ampicillin resistance gene and the new restriction sites (EcoRI, XmaI and SmaI) when used for cloning, give rise to tetracycline resistant transformants.
摘要
构建了一种大肠杆菌质粒载体pUN121，它可对携带DNA插入片段的转化体进行阳性筛选。携带DNA插入片段的转化体的阳性筛选。该载体基于质粒pTR262（罗伯茨等人，《基因》，第12卷，（1980年），123 - 127页），其中四环素抗性基因受噬菌体λ cI基因编码的阻遏蛋白的转录控制。利用包括寡核苷酸介导的诱变在内的体外重组技术对该质粒进行了重排，以产生一个较小的质粒（4.4 kb），除了独特的HindIII和BclI位点外，还具有EcoRI、XmaI和SmaI的独特克隆位点。该质粒具有功能性氨苄青霉素抗性基因，当用于克隆时，新的限制酶切位点（EcoRI、XmaI和SmaI）会产生四环素抗性转化体。
相似文献
1
An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis.通过寡核苷酸介导诱变构建的改良型阳性选择质粒载体。
Nucleic Acids Res. 1983 Nov 25;11(22):8019-30. doi: 10.1093/nar/11.22.8019.
2
A plasmid cloning vehicle allowing a positive selection for inserted fragments.一种能对插入片段进行阳性筛选的质粒克隆载体。
Gene. 1980 Dec;12(1-2):123-7. doi: 10.1016/0378-1119(80)90022-0.
3
Construction of a new Escherichia coli-Saccharomyces cerevisiae shuttle plasmid cloning vector allowing positive selection for cloned fragments.构建一种新型大肠杆菌-酿酒酵母穿梭质粒克隆载体，可对克隆片段进行阳性筛选。
Folia Microbiol (Praha). 1992;37(3):193-8. doi: 10.1007/BF02933146.
4
A selective lambda phage cloning vector with automatic excision of the insert in a plasmid.一种选择性λ噬菌体克隆载体，可在质粒中自动切除插入片段。
Gene. 1992 Oct 21;120(2):135-41. doi: 10.1016/0378-1119(92)90086-5.
5
Cloning of complementary DNA inserts from phage DNA directly into plasmid vector.将噬菌体DNA中的互补DNA插入片段直接克隆到质粒载体中。
Methods Enzymol. 1992;216:508-16. doi: 10.1016/0076-6879(92)16046-m.
6
Generalized and mobilizable positive-selection cloning vectors.通用且可移动的阳性选择克隆载体。
Gene. 1994 May 27;143(1):145-6. doi: 10.1016/0378-1119(94)90620-3.
7
A new selective phage cloning vector, lambda 2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI.一种新的选择性噬菌体克隆载体λ2001，带有XbaI、BamHI、HindIII、EcoRI、SstI和XhoI的酶切位点。
Gene. 1984 Dec;32(1-2):217-24. doi: 10.1016/0378-1119(84)90049-0.
8
A small mobilizable IncP group plasmid vector packageable into bacteriophage lambda capsids in vitro.一种小型可移动的IncP组质粒载体，可在体外包装到噬菌体λ衣壳中。
Plasmid. 1985 Jan;13(1):70-4. doi: 10.1016/0147-619x(85)90057-5.
9
A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.一种源自pSC101的质粒，与其他常用克隆载体无序列同源性。
Gene. 1984 Nov;31(1-3):165-71. doi: 10.1016/0378-1119(84)90207-5.
10
A novel ColE1::Tn3 plasmid vector that allows direct selection of hybrid clones in E. coli.一种新型的ColE1::Tn3质粒载体，可在大肠杆菌中直接筛选杂交克隆。
Gene. 1980 Feb;8(3):301-14. doi: 10.1016/0378-1119(80)90006-2.
引用本文的文献
1
Construction of a highly error-prone DNA polymerase for developing organelle mutation systems.构建一个高易错 DNA 聚合酶，用于开发细胞器突变系统。
Nucleic Acids Res. 2020 Dec 2;48(21):11868-11879. doi: 10.1093/nar/gkaa929.
2
Phages Isolated from Industrial Dairy Plants-from Physiological to Genomic Analyses.从生理学到基因组分析：从工业乳制品厂中分离的噬菌体。
Viruses. 2020 Mar 3;12(3):280. doi: 10.3390/v12030280.
3
Auxin-induced mRNA species in tobacco cell cultures.烟草原生细胞培养物中生长素诱导的 mRNA 种类。
Plant Mol Biol. 1987 Mar;10(2):145-57. doi: 10.1007/BF00016152.
4
Cytokinin stress changes the developmental regulation of several defence-related genes in tobacco.细胞分裂素胁迫改变了烟草中几个与防御相关基因的发育调控。
EMBO J. 1987 Dec 1;6(12):3579-83. doi: 10.1002/j.1460-2075.1987.tb02688.x.
5
Nonbioluminescent strains of Photobacterium phosphoreum produce the cell-to-cell communication signal N-(3-Hydroxyoctanoyl)homoserine lactone.非发光型磷光杆菌菌株产生细胞间通讯信号N-(3-羟基辛酰基)高丝氨酸内酯。
Appl Environ Microbiol. 2005 Apr;71(4):2113-20. doi: 10.1128/AEM.71.4.2113-2120.2005.
6
Altering a gene involved in nuclear distribution increases the repeat-induced point mutation process in the fungus Podospora anserina.改变一个与核分布有关的基因会增加真菌粗糙脉孢菌中的重复诱导点突变过程。
Genetics. 2004 May;167(1):151-9. doi: 10.1534/genetics.167.1.151.
7
2,4-Dichlorophenoxyacetic Acid and Related Chlorinated Compounds Inhibit Two Auxin-Regulated Type-III Tobacco Glutathione S-Transferases.2,4-二氯苯氧乙酸及相关氯化化合物抑制两种生长素调节的III型烟草谷胱甘肽S-转移酶。
Plant Physiol. 1995 Apr;107(4):1139-1146. doi: 10.1104/pp.107.4.1139.
8
TCA1, a single nuclear-encoded translational activator specific for petA mRNA in Chlamydomonas reinhardtii chloroplast.TCA1，一种针对莱茵衣藻叶绿体中petA mRNA的单核编码翻译激活因子。
Genetics. 2001 Sep;159(1):119-32. doi: 10.1093/genetics/159.1.119.
9
Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability.嗜糖栖棒杆菌sodA基因的克隆及超氧化物歧化酶在细胞活力中的作用
J Bacteriol. 2001 Feb;183(4):1284-95. doi: 10.1128/JB.2001.183.4.1284-1295.2001.
10
Cloning of the malic enzyme gene from Corynebacterium glutamicum and role of the enzyme in lactate metabolism.谷氨酸棒杆菌苹果酸酶基因的克隆及其在乳酸代谢中的作用。
Appl Environ Microbiol. 2000 Jul;66(7):2981-7. doi: 10.1128/AEM.66.7.2981-2987.2000.
本文引用的文献
1
Synthesis of mixed oligodeoxyribonucleotides following the solid phase method.采用固相法合成混合寡聚脱氧核糖核苷酸。
Nucleic Acids Res. 1982 May 25;10(10):3291-301. doi: 10.1093/nar/10.10.3291.
2
Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent phi X174 mutant, with a specific nucleotide deletion, at very high efficiency.使用寡脱氧核糖核苷酸进行位点特异性诱变：高效分离出具有特定核苷酸缺失的表型沉默的φX174突变体。
Gene. 1980 Dec;12(1-2):129-37. doi: 10.1016/0378-1119(80)90023-2.
3
A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.一种用于克隆DNA区域特异性和链特异性诱变的快速高效方法。
EMBO J. 1982;1(4):433-7. doi: 10.1002/j.1460-2075.1982.tb01187.x.
4
Positive-selection cloning vehicle useful for overproduction of hybrid proteins.用于过量生产杂合蛋白的阳性选择克隆载体。
J Bacteriol. 1983 May;154(2):1005-8. doi: 10.1128/jb.154.2.1005-1008.1983.
5
Direct selection of recombinant plasmids in Bacillus subtilis.枯草芽孢杆菌中重组质粒的直接筛选
Gene. 1982 Dec;20(3):459-69. doi: 10.1016/0378-1119(82)90215-3.
6
Expression of the Eco RI restriction-modification system and the construction of positive-selection cloning vectors.Eco RI限制修饰系统的表达及正选择克隆载体的构建。
Gene. 1982 Dec;20(2):219-29. doi: 10.1016/0378-1119(82)90041-5.
7
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
8
A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids.一种用于直接筛选携带重组质粒菌株的质粒克隆载体。
Gene. 1981 Oct;15(1):99-102. doi: 10.1016/0378-1119(81)90108-6.
9
Synthesis of oligodeoxyribonucleotides on silica gel support.硅胶载体上寡脱氧核糖核苷酸的合成。
Nucleic Acids Res. 1981 Jun 25;9(12):2807-17. doi: 10.1093/nar/9.12.2807.
10
Plasmid vectors for positive selection of DNA inserts controlled by the lambda pL promoter, repressor and antitermination function.用于由λ pL启动子、阻遏物和抗终止功能控制的DNA插入片段阳性选择的质粒载体。
Gene. 1981 Apr;13(3):289-98. doi: 10.1016/0378-1119(81)90033-0.
Zotero 插件