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酿酒酵母中高效启动子序列的保守性。

Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.

作者信息

Dobson M J, Tuite M F, Roberts N A, Kingsman A J, Kingsman S M, Perkins R E, Conroy S C, Fothergill L A

出版信息

Nucleic Acids Res. 1982 Apr 24;10(8):2625-37. doi: 10.1093/nar/10.8.2625.

DOI:10.1093/nar/10.8.2625
PMID:6281737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320638/
Abstract

The position of the yeast phosphoglycerate kinase (PGK) gene has been mapped on a 2.95kb Hind III fragment. We have determined the nucleotide sequence of the 5' flanking region and compared this sequence with those from 16 other yeast genes. PGK, like all other yeast genes has an adenine residue at position -3. It has two possible TATA boxes at positions -114 and -152 and a CAAT box at -129. In addition we have defined a structure at position -63 to -39 that is common to all yeast genes that encode an abundant RNA. This structure is a CT-rich block followed, about 10 nucleotides later, by the sequence CAAG.

摘要

酵母磷酸甘油酸激酶(PGK)基因的位置已定位在一个2.95kb的Hind III片段上。我们测定了其5'侧翼区域的核苷酸序列,并将该序列与其他16个酵母基因的序列进行了比较。与所有其他酵母基因一样,PGK在-3位置有一个腺嘌呤残基。它在-114和-152位置有两个可能的TATA框,在-129位置有一个CAAT框。此外,我们在-63至-39位置定义了一种结构,该结构是所有编码丰富RNA的酵母基因所共有的。这种结构是一个富含CT的区域,大约10个核苷酸后是序列CAAG。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb15/320638/b5b819e5c36e/nar00377-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb15/320638/b5b819e5c36e/nar00377-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb15/320638/b5b819e5c36e/nar00377-0149-a.jpg

相似文献

1
Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.酿酒酵母中高效启动子序列的保守性。
Nucleic Acids Res. 1982 Apr 24;10(8):2625-37. doi: 10.1093/nar/10.8.2625.
2
Characterisation of sequences required for RNA initiation from the PGK promoter of Saccharomyces cerevisiae.酿酒酵母磷酸甘油酸激酶(PGK)启动子RNA起始所需序列的表征
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The primary structure of the Saccharomyces cerevisiae gene for 3-phosphoglycerate kinase.酿酒酵母3-磷酸甘油酸激酶基因的一级结构。
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Effect of leader primary structure on the translational efficiency of phosphoglycerate kinase mRNA in yeast.酵母中前导序列一级结构对磷酸甘油酸激酶mRNA翻译效率的影响。
Yeast. 1990 Nov-Dec;6(6):473-82. doi: 10.1002/yea.320060604.

引用本文的文献

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Unraveling the Mechanical Unfolding Pathways of a Multidomain Protein: Phosphoglycerate Kinase.解析多结构域蛋白:磷酸甘油酸激酶的机械解折叠途径。
Biophys J. 2018 Jul 3;115(1):46-58. doi: 10.1016/j.bpj.2018.05.028.
2
Multiple novel promoter-architectures revealed by decoding the hidden heterogeneity within the genome.通过解码基因组内隐藏的异质性揭示了多种新型启动子结构。
Nucleic Acids Res. 2014 Nov 10;42(20):12388-403. doi: 10.1093/nar/gku924. Epub 2014 Oct 17.
3
Hygromycin B resistance as dominant selectable marker in yeast.潮霉素 B 抗性作为酵母中的显性选择标记。

本文引用的文献

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Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.侧翼核苷酸在真核生物核糖体识别AUG起始密码子中的可能作用。
Nucleic Acids Res. 1981 Oct 24;9(20):5233-52. doi: 10.1093/nar/9.20.5233.
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The sequence of the DNAs coding for the mating-type loci of Saccharomyces cerevisiae.酿酒酵母交配型基因座的编码DNA序列。
Cell. 1981 Nov;27(1 Pt 2):15-23. doi: 10.1016/0092-8674(81)90356-1.
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Nucleic Acids Res. 2013 Jun;41(11):5569-81. doi: 10.1093/nar/gkt256. Epub 2013 Apr 17.
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Mol Genet Genomics. 2011 Aug;286(2):143-59. doi: 10.1007/s00438-011-0634-z. Epub 2011 Jul 13.
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Nucleic Acids Res. 2006 May 17;34(9):2587-97. doi: 10.1093/nar/gkl280. Print 2006.
9
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Proc Natl Acad Sci U S A. 1985 Jan;82(2):334-8. doi: 10.1073/pnas.82.2.334.
10
The promoter region of the arg3 gene in Saccharomyces cerevisiae: nucleotide sequence and regulation in an arg3-lacZ gene fusion.酿酒酵母中arg3基因的启动子区域:arg3 - lacZ基因融合体中的核苷酸序列及调控
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4
Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae.酿酒酵母中分散重复序列的序列变异。
J Mol Biol. 1981 Feb 5;145(4):619-32. doi: 10.1016/0022-2836(81)90306-5.
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Histone H2B genes of yeast encode two different proteins.酵母的组蛋白H2B基因编码两种不同的蛋白质。
Cell. 1980 Dec;22(3):799-805. doi: 10.1016/0092-8674(80)90556-5.
6
The actin gene in yeast Saccharomyces cerevisiae: 5' and 3' end mapping, flanking and putative regulatory sequences.酿酒酵母中的肌动蛋白基因:5'和3'末端定位、侧翼及推定的调控序列。
Nucleic Acids Res. 1981 Dec 11;9(23):6339-50. doi: 10.1093/nar/9.23.6339.
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Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.异-1-细胞色素c基因体内转录所需序列的缺失图谱分析。
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J Biol Chem. 1981 Feb 10;256(3):1385-95.
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Isolation and characterization of the yeast 3-phosphoglycerokinase gene (PGK) by an immunological screening technique.通过免疫筛选技术分离和鉴定酵母3-磷酸甘油酸激酶基因(PGK)。
J Biol Chem. 1980 Dec 25;255(24):12073-80.
10
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Nucleic Acids Res. 1980 Apr 25;8(8):1731-43. doi: 10.1093/nar/8.8.1731.