Dobson M J, Tuite M F, Roberts N A, Kingsman A J, Kingsman S M, Perkins R E, Conroy S C, Fothergill L A
Nucleic Acids Res. 1982 Apr 24;10(8):2625-37. doi: 10.1093/nar/10.8.2625.
The position of the yeast phosphoglycerate kinase (PGK) gene has been mapped on a 2.95kb Hind III fragment. We have determined the nucleotide sequence of the 5' flanking region and compared this sequence with those from 16 other yeast genes. PGK, like all other yeast genes has an adenine residue at position -3. It has two possible TATA boxes at positions -114 and -152 and a CAAT box at -129. In addition we have defined a structure at position -63 to -39 that is common to all yeast genes that encode an abundant RNA. This structure is a CT-rich block followed, about 10 nucleotides later, by the sequence CAAG.
酵母磷酸甘油酸激酶(PGK)基因的位置已定位在一个2.95kb的Hind III片段上。我们测定了其5'侧翼区域的核苷酸序列,并将该序列与其他16个酵母基因的序列进行了比较。与所有其他酵母基因一样,PGK在-3位置有一个腺嘌呤残基。它在-114和-152位置有两个可能的TATA框,在-129位置有一个CAAT框。此外,我们在-63至-39位置定义了一种结构,该结构是所有编码丰富RNA的酵母基因所共有的。这种结构是一个富含CT的区域,大约10个核苷酸后是序列CAAG。