L. 叶的抗自由基和黄嘌呤氧化酶抑制活性评估以及通过液相色谱-四极杆飞行时间串联质谱法和分子对接方法对生物活性成分的初步鉴定。
Antiradical and Xanthine Oxidase Inhibitory Activity Evaluations of L. Leaves and Tentative Identification of Bioactive Constituents through LC-QTOF-MS/MS and Molecular Docking Approach.
作者信息
Ahmed Qamar Uddin, Alhassan Alhassan Muhammad, Khatib Alfi, Shah Syed Adnan Ali, Hasan Muhammad Mahmudul, Sarian Murni Nazira
机构信息
Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Pahang DM, Kuantan 25200, Malaysia.
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto P.M.B. 2346, Nigeria.
出版信息
Antioxidants (Basel). 2018 Oct 8;7(10):137. doi: 10.3390/antiox7100137.
The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of leaves. Hence, crude methanolic leaves extract and its resultant fractions, namely hexane, chloroform, and n-butanol were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC (4.14 ± 0.21 μg/mL) ( < 0.05), as well as xanthine oxidase inhibitory activity with IC (64.84 ± 3.93 μg/mL) ( < 0.05). Afzelechin 3--alpha-l-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction.
本研究的目的是探究树叶的抗自由基和黄嘌呤氧化酶抑制作用。因此，对粗甲醇树叶提取物及其所得馏分，即己烷、氯仿和正丁醇，进行了2,2-二苯基-1-苦基肼基（DPPH）自由基清除效果和黄嘌呤氧化酶抑制活性的评估。通过液相色谱-四极杆飞行时间串联质谱（LC-QTOF-MS/MS）和分子对接方法初步鉴定了活性成分。粗甲醇树叶提取物的正丁醇馏分表现出显著的DPPH自由基清除效果，IC50为（4.14±0.21μg/mL）（P<0.05），同时具有黄嘌呤氧化酶抑制活性，IC50为（64.84±3.93μg/mL）（P<0.05）。初步鉴定出Afzelechin 3--α-L-鼠李吡喃糖苷和黄瓜苷A可能是有助于正丁醇馏分抗氧化活性的代谢产物。
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