Danilevicz Monica F, Moharana Kanhu C, Venancio Thiago M, Franco Luciana O, Cardoso Sérgio R S, Cardoso Mônica, Thiebaut Flávia, Hemerly Adriana S, Prosdocimi Francisco, Ferreira Paulo C G
Laboratório de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-599, Brazil.
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro 28013-602, Brazil.
Noncoding RNA. 2018 Oct 8;4(4):27. doi: 10.3390/ncrna4040027.
Long non-coding RNAs (lncRNAs) are involved in multiple regulatory pathways and its versatile form of action has disclosed a new layer in gene regulation. LncRNAs have their expression levels modulated during plant development, and in response to stresses with tissue-specific functions. In this study, we analyzed lncRNA from leaf samples collected from the legume Desf. (copaíba) present in two divergent ecosystems: Cerrado (CER; Ecological Station of Botanical Garden in Brasília, Brazil) and Atlantic Rain Forest (ARF; Rio de Janeiro, Brazil). We identified 8020 novel lncRNAs, and they were compared to seven Fabaceae genomes and transcriptomes, to which 1747 and 2194 copaíba lncRNAs were mapped, respectively, to at least one species. The secondary structures of the lncRNAs that were conserved and differentially expressed between the populations were predicted using in silico methods. A few selected lncRNA were confirmed by RT-qPCR in the samples from both biomes; Additionally, the analysis of the lncRNA sequences predicted that some might act as microRNA (miRNA) targets or decoys. The emerging studies involving lncRNAs function and conservation have shown their involvement in several types of biotic and abiotic stresses. Thus, the conservation of lncRNAs among Fabaceae species considering their rapid turnover, suggests they are likely to have been under functional conservation pressure. Our results indicate the potential involvement of lncRNAs in the adaptation of . in two different biomes.
长链非编码RNA（lncRNAs）参与多种调控途径，其多样的作用形式揭示了基因调控的新层面。lncRNAs在植物发育过程中表达水平受到调控，并在响应胁迫时具有组织特异性功能。在本研究中，我们分析了从豆科植物 （苦配巴）的叶片样本中提取的lncRNAs，该植物存在于两个不同的生态系统中：塞拉多（CER；巴西巴西利亚植物园生态站）和大西洋雨林（ARF；巴西里约热内卢）。我们鉴定出8020个新的lncRNAs，并将它们与七个豆科植物基因组和转录组进行比较，分别有1747个和2194个苦配巴lncRNAs至少映射到一个物种上。使用计算机方法预测了种群间保守且差异表达的lncRNAs的二级结构。通过RT-qPCR在来自两个生物群落的样本中证实了一些选定的lncRNA；此外，对lncRNA序列的分析预测，一些lncRNA可能作为微小RNA（miRNA）的靶标或诱饵。涉及lncRNAs功能和保守性的新兴研究表明它们参与了几种类型的生物和非生物胁迫。因此，考虑到lncRNAs的快速更新，豆科植物物种间lncRNAs的保守性表明它们可能一直处于功能保守压力之下。我们的结果表明lncRNAs可能参与了 在两个不同生物群落中的适应性。
