Grogan E, Jenson H, Countryman J, Heston L, Gradoville L, Miller G
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1332-6. doi: 10.1073/pnas.84.5.1332.
An Epstein-Barr viral gene (ZEBRA) is identified that, in human lymphoblastoid cells, activates a switch causing the virus to shift from the latent to the replicative phase of its life cycle. We have shown that a 2.7-kilobase-pair rearranged Epstein-Barr virus DNA fragment of this gene (BamHI fragment WZhet) induced transient expression of viral replicative antigens and polypeptides when it was transfected into a somatic cell hybrid, which was derived from the fusion of an epithelial line cell with a Burkitt lymphoma cell. We now show that this rearranged WZhet fragment, when introduced stably into lymphoblastoid cells, will activate expression of the complete viral replicative cycle in 1-10% of the lymphoblastoid cells, leading to production of biologically active virions that can immortalize primary lymphocytes. The transfected plasmid appears to be regulated in a manner analogous to the complete Epstein-Barr virus genome.
一种爱泼斯坦-巴尔病毒基因（ZEBRA）被鉴定出来，在人类淋巴母细胞中，它激活一个开关，使病毒从其生命周期的潜伏阶段转变为复制阶段。我们已经表明，该基因的一个2.7千碱基对重排的爱泼斯坦-巴尔病毒DNA片段（BamHI片段WZhet），当被转染到一个体细胞杂种中时，能诱导病毒复制抗原和多肽的瞬时表达，该体细胞杂种是由一个上皮系细胞与一个伯基特淋巴瘤细胞融合而成。我们现在表明，这个重排的WZhet片段，当被稳定地导入淋巴母细胞时，将在1%至10%的淋巴母细胞中激活完整病毒复制周期的表达，导致产生能使原代淋巴细胞永生化的生物活性病毒粒子。转染的质粒似乎以类似于完整爱泼斯坦-巴尔病毒基因组的方式受到调控。
