School of Chemical Engineering, Northwest University, Xi'an, 710069, Shaanxi, China.
College of Food Science and Technology, Northwest University, Xi'an, 710069, Shaanxi, China.
Biotechnol Prog. 2019 Jan;35(1):e2726. doi: 10.1002/btpr.2726. Epub 2018 Oct 17.
The synthesis of nonnatural phospholipid, phosphatidylhydroxybutyrate (PB), was firstly introduced by phospholipase D (PLD)-mediated transphosphatidylation of phosphatidylcholine (PC) with sodium γ-hydroxybutyrate (NaGHB) in the aqueous-solid system. Nanoscale silicon dioxide (NSD) was employed as a carrier to provide an "artificial interphase" between PC and PLD. Special attention has been paid to the effect of the PC coverage on the surface area of hybrids of NSD-PC, the PC loading and the yield of PB. Results indicated that the highest PC loading of 98.3% and the highest PB yield of 97.3% were achieved. In addition, the free PLD in the aqueous-solid system showed the greater stability and pH tolerance than that in the traditional liquid-liquid system. The operational stability of free PLD solution was investigated. The yield of PB remained 70.7% after being used for five batches. The authors provide a new idea for drug design and the potential source of PB for medical experiments. PB is a potential drug and may have the excellent performance in the treatment of central nervous system's diseases. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2726, 2019.
非天然磷脂，即磷脂酰羟基丁酸（PB）的合成，最初是通过磷脂酶 D（PLD）介导的在水-固体系中，以磷酸胆碱（PC）与γ-羟基丁酸钠（NaGHB）进行的转磷酸化反应实现的。纳米二氧化硅（NSD）被用作载体，为 PC 和 PLD 之间提供“人工界面”。特别关注了 PC 覆盖度对 NSD-PC 混合体的表面积、PC 负载量和 PB 产率的影响。结果表明，实现了最高的 PC 负载量 98.3%和最高的 PB 产率 97.3%。此外，水-固体系中的游离 PLD 比传统的液-液体系中的游离 PLD 具有更好的稳定性和 pH 耐受性。考察了游离 PLD 溶液的操作稳定性。在使用五批后，PB 的产率仍保持在 70.7%。作者为药物设计提供了一个新的思路，并为医学实验提供了 PB 的潜在来源。PB 是一种有潜力的药物，在治疗中枢神经系统疾病方面可能具有优异的性能。 © 2018 美国化学工程师协会生物技术进展，35：e2726，2019。
