Aleskerova L E, Alenina K A, Efremnko E N, Ismailov A D
Mikrobiologiia. 2017 Mar-Apr;86(2):201-8.
Immobilization of photobacteria in the cryogel of polyvinyl alcohol (PVA) was carried out. Immobilization was found to result in increased intensity and stability of bioluminescence. The elements determining the stability of bioluminescence were investigated. Selection of the strain was found to be of the highest importance. Among immobilized cells, Photobacterium phosphoreum exhibited the most intense and prolonged light emission, while Vibrio harveyi showed the least one. The technological procedures for cryogenic immobilization of photobacteria were determined. The role of the environment of gel formation in the preservation of the bioluminescence activity was determined. In the gels formed in rich medium for submerged cultivation of photobacteria, almost 100% luminescence activity was preserved, while light emission was considerably prolonged. Bioluminescence intensity of the preparations was shown to depend significantly on pH of the incubation medium. The pH shift to acidic values during prolonged incubation of immobilized cells was shown to be one of the factors of bioluminescence quenching. The stress effects of cryogenic immobilization were found to have an insignificant effect on the temperature profile of bioluminescence. Decreased reduction rate of the luciferase flavin substrate was shown to be a possible reason for bioluminescence quenching.
进行了发光细菌在聚乙烯醇（PVA）冷冻凝胶中的固定化。发现固定化导致生物发光强度增加和稳定性提高。研究了决定生物发光稳定性的因素。发现菌株的选择至关重要。在固定化细胞中，磷光杆菌发出的光最强烈且持续时间最长，而哈维弧菌发出的光最少。确定了发光细菌低温固定化的技术程序。确定了凝胶形成环境在生物发光活性保存中的作用。在用于发光细菌深层培养的丰富培养基中形成的凝胶中，几乎保留了100%的发光活性，同时发光持续时间显著延长。制剂的生物发光强度显示出明显依赖于孵育培养基的pH值。固定化细胞长时间孵育期间pH值向酸性值的变化被证明是生物发光猝灭的因素之一。发现低温固定化的应激效应对生物发光的温度曲线影响不大。荧光素酶黄素底物还原率降低被证明是生物发光猝灭的一个可能原因。
