Efremenko Elena N, Maslova Olga V, Kholstov Alexander V, Senko Olga V, Ismailov Anvar D
Lomonosov Moscow State University, Moscow, Russia.
Luminescence. 2016 Sep;31(6):1283-9. doi: 10.1002/bio.3104. Epub 2016 Feb 11.
We demonstrated the possibility of long-term and efficient application of a biosensitive element (BE) in the form of Photobacterium phosphoreum photobacteria immobilized in poly(vinyl alcohol) (PVA) cryogel for detecting various ecotoxicants (Zn(2) (+) , Cu(2) (+) , Hg(2) (+) , Pb(2) (+) , 2,4-dichlorophenoxyacetic acid, 2,6-dimethylphenol, pentachlorophenol, coumaphos, malathion, chlorpyrifos and methyl parathion) in flow-through media. The range of detectable concentrations of ecotoxicants was determined at 1 × 10(-8) to 1 × 10(-4) M for heavy metal ions and at 1 × 10(-8) to 1 × 10(-5) M for phenol derivatives and organophosphorus pesticides. Immobilized cells of photobacteria quantitatively reacted with these ecotoxicants; cell sensitivity exhibited no flow rate dependence in the range from 45 to 180 mL/h. At a constant concentration of ecotoxicant in the flow, the bioluminescence quenching profile of immobilized cells demonstrated an integral response. The BE could remain in a flow-through medium for at least 10 days while retaining 95% of luminescent activity in the absence of ecotoxicants. The BE tested in this work was demonstrated to have a long shelf life (> 60 weeks) at -80°C without changes in the baseline level of bioluminescence. Copyright © 2016 John Wiley & Sons, Ltd.
我们证明了以固定在聚乙烯醇（PVA）冷冻凝胶中的费氏弧菌光细菌形式存在的生物敏感元件（BE）可长期有效地应用于检测流通介质中的各种生态毒物（锌离子、铜离子、汞离子、铅离子、2,4-二氯苯氧乙酸、2,6-二甲基苯酚、五氯苯酚、蝇毒磷、马拉硫磷、毒死蜱和甲基对硫磷）。确定了生态毒物的可检测浓度范围，重金属离子为1×10⁻⁸至1×10⁻⁴ M，酚类衍生物和有机磷农药为1×10⁻⁸至1×10⁻⁵ M。固定化的光细菌细胞与这些生态毒物发生定量反应；细胞敏感性在45至180 mL/h的流速范围内不显示流速依赖性。在流动中生态毒物浓度恒定的情况下，固定化细胞的生物发光猝灭曲线呈现出整体响应。该生物敏感元件可在流通介质中保留至少10天，在不存在生态毒物的情况下保持95%的发光活性。在这项工作中测试的生物敏感元件在-80°C下显示出较长的保质期（>60周），生物发光基线水平无变化。版权所有©2016约翰威立父子有限公司。
