Kuhn R J, Wimmer E, Semler B L
Virology. 1987 Apr;157(2):560-4. doi: 10.1016/0042-6822(87)90302-3.
The introduction of a cDNA copy of poliovirus type 1 (Mahoney) into cultured primate cells results in the production of infectious virus. The level of infectious virus can be increased by incorporation of eukaryotic signals of transcription and replication. We have utilized the SV40 DNA sequence coding for the early and late promoters, the SV40 origin of replication, and the enhancer elements, along with the cDNA of poliovirus, to determine the important parameters for the level of infectious virus produced following transfection. Although plasmid replication increases the level of infectivity, the major determinant of infectivity is promoter activity.
将脊髓灰质炎病毒1型（Mahoney株）的互补DNA（cDNA）拷贝导入培养的灵长类细胞中会产生传染性病毒。通过掺入真核转录和复制信号可提高传染性病毒的水平。我们利用编码早期和晚期启动子的猴病毒40（SV40）DNA序列、SV40复制起点和增强子元件，以及脊髓灰质炎病毒的cDNA，来确定转染后产生的传染性病毒水平的重要参数。虽然质粒复制会提高感染性水平，但感染性的主要决定因素是启动子活性。
