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携带脊髓灰质炎病毒互补脱氧核糖核酸的高传染性质粒能够在转染的猿猴细胞中复制。

Highly infectious plasmids carrying poliovirus cDNA are capable of replication in transfected simian cells.

作者信息

Kean K M, Wychowski C, Kopecka H, Girard M

出版信息

J Virol. 1986 Aug;59(2):490-3. doi: 10.1128/JVI.59.2.490-493.1986.

Abstract

We examined events leading to production of infectious poliovirus upon transfection of simian cells with plasmids carrying poliovirus cDNA and simian virus 40 transcription and replication signals. The nature of the simian virus 40 promoter upstream from the poliovirus cDNA had no influence on its infectivity. A high specific infectivity was correlated with plasmid replication, dependent on expression of T antigen either encoded by the plasmid or present in host cells (COS-1).

摘要

我们研究了用携带脊髓灰质炎病毒cDNA以及猿猴病毒40转录和复制信号的质粒转染猴细胞后导致产生传染性脊髓灰质炎病毒的一系列事件。脊髓灰质炎病毒cDNA上游的猿猴病毒40启动子的性质对其感染性没有影响。高特异性感染性与质粒复制相关,这取决于由质粒编码或存在于宿主细胞(COS-1)中的T抗原的表达。

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