Wiegand V, Tracy S, Chapman N, Wucherpfennig C
Abteilung Kardiologie und Pulmonologie, Universität Göttingen.
Klin Wochenschr. 1990 Sep 14;68(18):914-20. doi: 10.1007/BF01649038.
In order to evaluate the role of enteroviral infections in end stage dilated cardiomyopathy, RNA was isolated from left ventricular myocardium of patients with dilated cardiomyopathy explanted during heart transplantation (n = 6) and from control hearts (n = 8), then probed using a dot blot procedure with two well-defined enteroviral cloned cDNA probes. One of the cardiomyopathic heart samples hybridized with the enteroviral probes, while RNA samples from the other diseased heart and the control heart demonstrated no hybridization. To verify further the enteroviral infection, a cDNA prepared from the positive heart RNA hybridized with Southern blotted coxsackievirus B3 and poliovirus 1 nucleotide sequences, while a control sample, which gave negative results in the dot blot, showed no hybridization to the enteroviral sequence. These results provide evidence for an enteroviral infection in certain patients with end stage dilated cardiomyopathy.
为了评估肠道病毒感染在终末期扩张型心肌病中的作用，从心脏移植时切除的扩张型心肌病患者（n = 6）的左心室心肌以及对照心脏（n = 8）中分离出RNA，然后使用斑点印迹法用两种明确的肠道病毒克隆cDNA探针进行检测。其中一个扩张型心肌病心脏样本与肠道病毒探针杂交，而其他患病心脏和对照心脏的RNA样本未显示杂交信号。为进一步验证肠道病毒感染，从阳性心脏RNA制备的cDNA与经Southern印迹的柯萨奇病毒B3和脊髓灰质炎病毒1核苷酸序列杂交，而在斑点印迹中呈阴性结果的对照样本未显示与肠道病毒序列杂交。这些结果为某些终末期扩张型心肌病患者存在肠道病毒感染提供了证据。
