Strang P, Stendahl U, Frankendal B, Lindgren A
Acta Radiol Oncol. 1986 Jul-Dec;25(4-6):249-54. doi: 10.3109/02841868609136414.
Flow cytometric measurements of the DNA content were performed in a prospective study of 167 women with invasive squamous cell carcinoma of the uterine cervix. Ploidy level and the proportion of S-phase cells were correlated to age, menopausal age, staging according to FIGO and histopathology. With increasing age a successive shift from a dominance of peri-diploid and peri-tetraploid values to marked aneuploidy was found. Peri-diploid and peri-tetraploid tumors were more often found in premenopausal than in post-menopausal women (p less than 0.001). The mean S-phase rate was significantly higher in women aged 60-89 years than in women aged 20-59 years (p less than 0.01). More aneuploid tumors were found in stages III and IV than in stages IB and II (p less than 0.01). The mean S-phase rate was higher in stages III and IV (20.8%) than in stages IB and II (17.2%) (p less than 0.01). No statistically significant correlation was shown between ploidy level and histopathology or between S-phase rate and histopathology. In 37 patients polyclonal tumors were found. The reproducibility of the method was good (r = 0.99).
对167例子宫颈浸润性鳞状细胞癌患者进行了前瞻性研究，采用流式细胞术测量DNA含量。倍体水平和S期细胞比例与年龄、绝经年龄、国际妇产科联合会（FIGO）分期及组织病理学相关。随着年龄的增加，发现从以亚二倍体和亚四倍体值为主逐渐转变为明显的非整倍体。亚二倍体和亚四倍体肿瘤在绝经前女性中比绝经后女性中更常见（p<0.001）。60 - 89岁女性的平均S期率显著高于20 - 59岁女性（p<0.01）。III期和IV期发现的非整倍体肿瘤比IB期和II期更多（p<0.01）。III期和IV期的平均S期率（20.8%）高于IB期和II期（17.2%）（p<0.01）。倍体水平与组织病理学之间或S期率与组织病理学之间未显示出统计学上的显著相关性。在37例患者中发现了多克隆肿瘤。该方法的可重复性良好（r = 0.99）。
