Strang P, Stendahl U, Sorbe B
Anticancer Res. 1986 Mar-Apr;6(2):171-5.
In a prospective study, the tumors of 167 patients with invasive squamous cell carcinomas of the uterine cervix were investigated by flow cytometric measurements of the DNA-content. In 37 patients (22%) 2 or more stemlines could be detected, indicating clonal heterogeneity. The proportion of multiple stemlines was equal in stages IB to III but significantly higher in stage IV (p less than 0.01). In 14 of the 37 polyclonal tumors the DNA-value of the second peak was twice the value of the first peak, suggesting possible polyploidization. For those tumors with evidence of polyploidization that had values in the diploid, tetraploid or octaploid region (n = 9) the mean age was 35.6 +/- 11.7 years and for grossly aneuploid tumors (n = 5) the mean age was 73 +/- 12.6 years. The differences of mean age between the two groups differed significantly (p less than 0.001). Flow cytometry proved to be a useful, rapid, and sensitive method for detection of multiple stemlines.
在一项前瞻性研究中，通过流式细胞术测量DNA含量，对167例浸润性子宫颈鳞状细胞癌患者的肿瘤进行了研究。在37例患者（22%）中可检测到2条或更多的干系，表明存在克隆异质性。多干系的比例在IB期至III期相等，但在IV期显著更高（p<0.01）。在37例多克隆肿瘤中的14例中，第二个峰的DNA值是第一个峰的两倍，提示可能存在多倍体化。对于那些有证据表明多倍体化且值处于二倍体、四倍体或八倍体区域的肿瘤（n=9），平均年龄为35.6±11.7岁，而对于明显非整倍体肿瘤（n=5），平均年龄为73±12.6岁。两组之间的平均年龄差异显著（p<0.001）。流式细胞术被证明是一种检测多干系的有用、快速且灵敏的方法。
