Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Shandong Universities, Taishan Medical College, Taian, Shandong, China.
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing, China.
Transbound Emerg Dis. 2019 Jan;66(1):592-598. doi: 10.1111/tbed.13037. Epub 2018 Oct 26.
H6 subtype avian influenza virus (AIV) was prevalent in poultry and could sporadically infect humans. Here, a total of 196 novel H6 AIVs isolated from poultry in eight provinces of China from 2014 to 2016 were phylogenetically characterized. Our analysis revealed that they could be divided into two clades in the Asian H6 HA lineage, A/wild duck/Shantou/2853/2003(H6N2) (ST2853-like) (85.7%) and A/duck/Shantou/339/2000(H6N2) (ST339-like) (14.3%), in which ST2853-like strains predominate. These novel strains belonged to the H6N6 (n = 165, 84.2%), H6N2 (n = 30, 15.3%), and H6N3 (n = 1, 0.51%) subtypes, which could be classified into 36 genotypes including 12 novel genotypes described in this study. In particular, several strains possessed the V190 and S228 mutations in HA (H3 numbering), which is critical for human receptor binding and identical to the human-derived strain A/Taiwan/2/2013(H6N1). Furthermore, 10.3% of the H6N6 isolates possessed the N6-∆11b (59-69) deletion. In summary, we describe phylogenetic and molecular characterizations of H6 AIVs in southern China and highlight the constant prevalence of H6 AIVs in poultry as well as adaptation to mammalian hosts.
H6 亚型禽流感病毒（AIV）在禽类中流行，并可偶发感染人类。本研究共对 2014 年至 2016 年期间从中国八个省份的禽类中分离的 196 株新型 H6 AIV 进行了系统发育特征分析。分析结果显示，它们可分为亚洲 H6 HA 谱系中的两个分支，A/wild duck/Shantou/2853/2003(H6N2)（ST2853 样）（85.7%）和 A/duck/Shantou/339/2000(H6N2)（ST339 样）（14.3%），其中 ST2853 样株占优势。这些新型毒株属于 H6N6（n=165，84.2%）、H6N2（n=30，15.3%）和 H6N3（n=1，0.51%）亚型，可分为 36 种基因型，包括本研究中描述的 12 种新型基因型。特别是，一些毒株的 HA（H3 编号）具有 V190 和 S228 突变，这对人类受体结合至关重要，与源自人类的 A/Taiwan/2/2013(H6N1)株相同。此外，10.3%的 H6N6 分离株具有 N6-∆11b（59-69）缺失。总之，本研究描述了中国南方 H6 AIV 的系统发育和分子特征，并强调了 H6 AIV 在禽类中的持续流行以及对哺乳动物宿主的适应。
