College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
Virol J. 2011 Oct 14;8:470. doi: 10.1186/1743-422X-8-470.
Although extensive data demonstrates that the majority of H6 duck isolates belonged to a single H6N2 virus lineage with a single gene constellation in southern China from 2000 to 2005, the prevalence of H6N2 virus in poultry in Eastern China is largely unknown.
Epidemiology revealed that H6N2 viruses were the most frequently detected influenza subtypes in live bird markets from 2002 to 2008 in Eastern China, but from 2009 onwards, they were replaced with novel H6N6 viruses. We phylogenetically and antigenically analyzed 42 H6 viruses isolated mainly in domestic ducks from 2002 to 2010 in Eastern China. Surprisingly, none of these isolates grouped with the previously described H6N2 viruses which belonged to a single H6N2 virus lineage with a single gene constellation in domestic ducks in southern China from 2000 to 2005. Two distinct hemagglutinin lineages were identified and they all underwent frequent reassortment with multiple virus subtypes from the natural gene pool, but few reassortants were persistent or prevalent.
Five subtypes of H6 influenza viruses (H6N1, H6N2, H6N5, H6N6 and H6N8) cocirculated in Eastern China, which form a significant part of the natural influenza virus reservoir in domestic ducks, and significant viral reassortment is still ongoing in this species.
尽管有大量数据表明，2000 年至 2005 年期间，中国南方的大多数 H6 鸭分离株属于单一的 H6N2 病毒谱系，具有单一的基因组合，但中国东部家禽中 H6N2 病毒的流行情况尚不清楚。
流行病学调查显示，2002 年至 2008 年期间，H6N2 病毒是中国东部活禽市场中最常检测到的流感亚型，但自 2009 年以来，它们被新型 H6N6 病毒所取代。我们对 2002 年至 2010 年期间主要从中国东部的家鸭中分离到的 42 株 H6 病毒进行了系统发育和抗原分析。令人惊讶的是，这些分离株均与 2000 年至 2005 年期间中国南方的单一 H6N2 病毒谱系中的先前描述的 H6N2 病毒不同。鉴定出了两个不同的血凝素谱系，它们都与来自天然基因库的多种病毒亚型频繁发生重配，但很少有重配体是持续或流行的。
在中国东部，五种 H6 流感病毒亚型（H6N1、H6N2、H6N5、H6N6 和 H6N8）共同流行，它们构成了家鸭中天然流感病毒库的重要组成部分，并且在该物种中仍在持续发生重要的病毒重配。
