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HT29人结肠腺癌细胞系中果糖2,6-二磷酸浓度的激素调控。α2-肾上腺素能激动剂可抵消血管活性肠肽的作用。

Hormonal control of fructose 2,6-bisphosphate concentration in the HT29 human colon adenocarcinoma cell line. Alpha 2-adrenergic agonists counteract effect of vasoactive intestinal peptide.

作者信息

Denis C, Paris H, Murat J C

出版信息

Biochem J. 1986 Nov 1;239(3):531-6. doi: 10.1042/bj2390531.

DOI:10.1042/bj2390531
PMID:3030263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147319/
Abstract

Vasoactive intestinal peptide (VIP) was found to cause a dose-dependent decrease in fructose 2,6-bisphosphatase concomitant with an increase in cyclic AMP in cultured HT29 cancer cells from human colon. The maximum effect was a 41% decrease obtained with 10 nM-VIP, and half-maximum effect was obtained with 0.75 nM-VIP. The effect of 2.5 nM-VIP was almost totally counteracted (i.e. fructose 2,6-bisphosphate concentration was restored) by either adrenaline (1 microM) or the alpha 2-adrenergic agonist UK-14304 (1 microM); the alpha 2-agonist clonidine (1 microM) was less efficient, since the VIP effect was decreased by 72% only. The adrenaline effect was totally antagonized by 1 microM-yohimbine. It is concluded that, in the HT29 cancer cells, the fructose 2,6-bisphosphate-producing system is sensitive to variations of cyclic AMP concentration and is under the dual control of VIP and alpha 2-adrenergic receptors.

摘要

研究发现，血管活性肠肽（VIP）可导致人结肠来源的HT29培养癌细胞中果糖2,6 - 二磷酸酶呈剂量依赖性降低，同时细胞内环磷酸腺苷（cAMP）水平升高。最大效应是10 nM - VIP导致果糖2,6 - 二磷酸酶降低41%，半最大效应是0.75 nM - VIP时出现。2.5 nM - VIP的效应几乎完全被肾上腺素（1 μM）或α2 - 肾上腺素能激动剂UK - 14304（1 μM）抵消（即果糖2,6 - 二磷酸浓度恢复）；α2 - 激动剂可乐定（1 μM）的效果较差，因为它只能使VIP的效应降低72%。1 μM育亨宾可完全拮抗肾上腺素的作用。研究得出结论，在HT29癌细胞中，果糖2,6 - 二磷酸生成系统对cAMP浓度变化敏感，且受VIP和α2 - 肾上腺素能受体的双重调控。

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本文引用的文献

1
Fructose 2,6-bisphosphate. Hormonal regulation and mechanism of its formation in liver.果糖-2,6-二磷酸。其在肝脏中的激素调节及形成机制
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2
Activity of enzymes related to carbohydrate metabolism in the HT 29 colon adenocarcinoma cell line and tumor.HT-29结肠腺癌细胞系及肿瘤中与碳水化合物代谢相关的酶活性
Int J Biochem. 1984;16(1):87-91. doi: 10.1016/0020-711x(84)90055-7.
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The interaction of fructose 2,6-bisphosphate and AMP with rat hepatic fructose 1,6-bisphosphatase.果糖2,6-二磷酸和AMP与大鼠肝脏果糖1,6-二磷酸酶的相互作用。
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Fructose 2,6-bisphosphate, the probably structure of the glucose- and glucagon-sensitive stimulator of phosphofructokinase.果糖2,6-二磷酸，可能是磷酸果糖激酶的葡萄糖和胰高血糖素敏感刺激物的结构。
Biochem J. 1980 Dec 15;192(3):897-901. doi: 10.1042/bj1920897.
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Growth-related enzymatic control of glycogen metabolism in cultured human tumor cells.培养的人肿瘤细胞中糖原代谢的生长相关酶促调控
Cancer Res. 1984 Jan;44(1):154-60.
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Effects of glucose and glucagon on the fructose 2,6-bisphosphate content of pancreatic islets and purified pancreatic B-cells. A comparison with isolated hepatocytes.葡萄糖和胰高血糖素对胰岛及纯化的胰腺β细胞中果糖2,6 -二磷酸含量的影响。与分离的肝细胞的比较。
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Annu Rev Biochem. 1983;52:617-53. doi: 10.1146/annurev.bi.52.070183.003153.
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