Hue L, Sherratt H S
Biochem J. 1986 Dec 15;240(3):765-9. doi: 10.1042/bj2400765.
Treatment of rats with hypoglycaemic doses of hypoglycin has been shown to abolish the relative detritiation of [2-3H,U-14C]glucose [Osmundsen, Billington, Taylor & Sherratt (1978) Biochem. J. 170, 337-342], indicating that both the Cori and the glucose/glucose 6-phosphate cycles were inhibited in vivo. This inhibition was confirmed and, in addition, it was shown that the conversion in vivo of both [14C]lactate and [14C]fructose into glucose was decreased after hypoglycin treatment. These results suggest that hypoglycin poisoning results in the inhibition in vivo of glucose-6-phosphatase activity, which participates in the overall inhibition of gluconeogenesis and hypoglycaemia. Clofibrate feeding apparently protected the rats against the inhibition of the fructose-to-glucose conversion by hypoglycin. However, in isolated hepatocytes prepared from hypoglycin-treated rats, the conversion of [14C]fructose into glucose and the recycling of [2-3H,U-14C]glucose were not different from that in control hepatocytes. This suggests that the inhibition was lost during preparation of the hepatocytes. The direct measurement of glucose-6-phosphatase activity showed that it was inhibited when measured in concentrated, but not dilute, homogenates prepared from hypoglycin-treated rats.
用低血糖剂量的降糖氨酸处理大鼠已显示可消除[2-³H，U-¹⁴C]葡萄糖的相对脱氚作用[奥斯芒森、比林顿、泰勒和谢拉特（1978年）《生物化学杂志》170卷，337 - 342页]，这表明科里循环和葡萄糖/葡萄糖6-磷酸循环在体内均受到抑制。这一抑制作用得到了证实，此外还表明，降糖氨酸处理后，[¹⁴C]乳酸和[¹⁴C]果糖在体内转化为葡萄糖的过程均减少。这些结果表明，降糖氨酸中毒导致体内葡萄糖-6-磷酸酶活性受到抑制，而该酶参与糖异生和低血糖的整体抑制过程。氯贝丁酯喂养显然保护大鼠免受降糖氨酸对果糖向葡萄糖转化的抑制。然而，在从经降糖氨酸处理的大鼠制备的分离肝细胞中，[¹⁴C]果糖转化为葡萄糖以及[2-³H，U-¹⁴C]葡萄糖的循环与对照肝细胞并无差异。这表明在肝细胞制备过程中抑制作用消失了。葡萄糖-6-磷酸酶活性的直接测量表明，在用经降糖氨酸处理的大鼠制备的浓缩匀浆（而非稀释匀浆）中测量时，该酶活性受到抑制。
