Huescas C G Y, Pereira R I, Prichula J, Azevedo P A, Frazzon J, Frazzon A P G
Programa de Pós-graduacao em Microbiologia Agricola e do Ambiente, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul - UFRGS, Sarmento Leite, 500, sala 216, CEP 90050-170, Porto Alegre, RS, Brasil.
Laboraorio de Gram-positive, Universidade Federal de Ciências da Saúde de Porto Alegre - UFCSPA, Sarmento Leite, 245, sala 204, CEP 90050-170, Porto Alegre, RS, Brasil.
Braz J Biol. 2019 Jul-Sep;79(3):460-465. doi: 10.1590/1519-6984.183375. Epub 2018 Oct 4.
The fidelity of the genomes is defended by mechanism known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems. Three Type II CRISPR systems (CRISPR1- cas, CRISPR2 and CRISPR3-cas) have been identified in enterococci isolates from clinical and environmental samples. The aim of this study was to observe the distribution of CRISPR1-cas, CRISPR2 and CRISPR3-cas in non-clinical strains of Enterococcus faecalis and Enterococcus faecium isolates from food and fecal samples, including wild marine animals. The presence of CRISPRs was evaluated by PCR in 120 enterococci strains, 67 E. faecalis and 53 E. faecium. It is the first report of the presence of the CRISPRs system in E. faecalis and E. faecium strains isolated from wild marine animal fecal samples. The results showed that in non-clinical strains, the CRISPRs were more frequently detected in E. faecalis than in E. faecium. And the frequencies of CRISPR1-cas and CRISPR2 were higher (60%) in E. faecalis strains isolated from animal feces, compared to food samples. Both strains showed low frequencies of CRISPR3-cas (8.95% and 1.88%). In conclusion, the differences in the habitats of enterococcal species may be related with the results observe in distribution of CRISPRs systems.
基因组的保真度由一种称为成簇规律间隔短回文重复序列（CRISPR）系统的机制来维护。在从临床和环境样本中分离出的肠球菌中，已鉴定出三种II型CRISPR系统（CRISPR1-cas、CRISPR2和CRISPR3-cas）。本研究的目的是观察CRISPR1-cas、CRISPR2和CRISPR3-cas在从食品和粪便样本（包括野生海洋动物）中分离出的粪肠球菌和屎肠球菌非临床菌株中的分布情况。通过PCR对120株肠球菌（67株粪肠球菌和53株屎肠球菌）进行CRISPRs检测。这是首次报道从野生海洋动物粪便样本中分离出的粪肠球菌和屎肠球菌菌株中存在CRISPRs系统。结果表明，在非临床菌株中，粪肠球菌中检测到CRISPRs的频率高于屎肠球菌。与食品样本相比，从动物粪便中分离出的粪肠球菌菌株中CRISPR1-cas和CRISPR2的频率更高（60%）。两种菌株中CRISPR3-cas的频率都较低（8.95%和1.88%）。总之，肠球菌物种栖息地的差异可能与观察到的CRISPRs系统分布结果有关。
