Mannella C A
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Experientia. 1990 Feb 15;46(2):137-45. doi: 10.1007/BF02027309.
Structural information about the channel in the mitochondrial outer membrane, derived from sequence analysis and electron microscopy of two-dimensional crystals, is summarized. A model for the channel is presented, consisting of a cylindrical beta-barrel that is formed by one or two 30-kDa polypeptides, with an alpha-carbon backbone diameter of 3.8 nm. The radial distributions of basic amino acids and lipid-contact regions on the projected cylinder are mapped relative to interchannel bonding sites inferred from channel packing in the arrays. Speculation on the kinds of conformational changes that the channel might undergo is also presented.
总结了通过对线粒体外膜通道的序列分析和二维晶体的电子显微镜观察所获得的结构信息。提出了该通道的模型，其由一个或两个30 kDa多肽形成的圆柱形β-桶组成，α-碳主链直径为3.8 nm。相对于从阵列中的通道堆积推断出的通道间结合位点，绘制了计划圆柱体上碱性氨基酸和脂质接触区域的径向分布。还对通道可能经历的构象变化类型进行了推测。
