Production of succinate by recombinant using acetate as the sole carbon source.

Acetate is a potential low-cost carbon source that can be generated by biological and chemical processes. In this study, deletion of encoding succinate dehydrogenase, encoding the isocitrate lyase regulator, and encoding the malic enzyme, and overexpression of encoding acetyl-CoA synthetase, encoding citrate synthase, and encoding aconitate hydratase in the wild-type MG1655 strain yielded the recombinant strain WCY-7, which could synthesize succinate from acetate. After 48 h batch fermentation, this strain accumulated 11.23 mM succinate from 50 mM sodium acetate. This work indicates that microbial fermentation using acetate as the sole carbon source may be a suitable route to produce high yields of the valuable chemicals.