Metabolic engineering of for the production of -succinyl-l-homoserine with high yield.

-succinyl-l-homoserine (OSH) is a promising platform chemical for the production of C4 chemicals with huge market potential which can be produced by fermentation from glucose. To construct a strain capable of producing OSH with high yield, the (encodes transcriptional repressor) and (encodes a subunit of dl-methionine transporter) were deleted in W3110 to obtain a strain ∆JI. Then, overexpression of (encodes bifunctional aspartate kinase/homoserine dehydrogenase II) and inactivation of (encodes cystathionine γ-synthase) were implemented in one step, and the OSH titer of the resulting strain ∆JIB Trc was dramatically increased to 7.30 g/L. The feedback regulation was further relieved by progressively overexpressing (encodes homoserine -succinyltransferase), (encodes l-methionine exporter), and (encodes bifunctional aspartate kinase/homoserine dehydrogenase I) to increase the metabolic flux from aspartate to OSH. The 100% rationally designed strain ∆JIB Trc/pTrc- -Trc- - produced 9.31 g/L OSH from 20 g/L glucose (0.466 g/g glucose) in batch fermentation, which represents the highest OSH yield from glucose reported to date. The culture profiles of the newly constructed strains were recorded to investigate their productive properties. The effects of l-methionine addition on the fermentation process of the optimal strain were also studied. Our results demonstrate that tuning the expression level of , inactivation of , and attenuation of feedback resistance of the crucial enzymes in the biosynthetic pathway are the key factors that impact the OSH production in .