Kumaresan Venkatesh, Pasupuleti Mukesh, Arasu Mariadhas Valan, Al-Dhabi Naif Abdullah, Arshad Aziz, Amin S M Nurul, Yusoff Fatimah Md, Arockiaraj Jesu
Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603 203, India.
Lab PCN 206, Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, Uttar Pradesh, 226 031, India.
Mol Biol Rep. 2018 Dec;45(6):2511-2523. doi: 10.1007/s11033-018-4418-y. Epub 2018 Oct 11.
Snakehead murrel, Channa striatus is an economically important aquatic species in Asia and are widely cultured and captured because of its nutritious and medicinal values. Their growth is predominantly affected by epizootic ulcerative syndrome (EUS) which is primarily caused by an oomycete fungus, Aphanomyces invadans. However, the molecular mechanism of immune response in murrel against this infection is still not clear. In this study, transcriptome technique was used to understand the molecular changes involved in C. striatus during A. invadans infection. RNA from the control (CF) and infected fish (IF) groups were sequenced using Illumina Hi-seq sequencing technology. For control group, 28,952,608 clean reads were generated and de novo assembly was performed to produce 60,753 contigs. For fungus infected group, 25,470,920 clean reads were obtained and assembled to produce 58,654 contigs. Differential gene expression analysis revealed that a total of 146 genes were up-regulated and 486 genes were down regulated. Most of the differentially expressed genes were involved in innate immune mechanism such as pathogen recognition, signalling and antimicrobial mechanisms. Interestingly, few adaptive immune genes, especially immunoglobulins were also significantly up regulated during fungal infection. Also, the results were validated by qRT-PCR analysis. These results indicated the involvement of various immune genes involved in both innate and adaptive immune mechanism during fungal infection in C. striatus which provide new insights into murrel immune mechanisms against A. invadans.
乌鳢，即条纹鳢，是亚洲一种具有重要经济价值的水生生物，因其营养价值和药用价值而被广泛养殖和捕捞。它们的生长主要受到流行性溃疡综合征（EUS）的影响，该综合征主要由一种卵菌纲真菌——入侵丝囊霉菌引起。然而，乌鳢针对这种感染的免疫反应分子机制仍不清楚。在本研究中，利用转录组技术来了解条纹鳢在感染入侵丝囊霉菌期间所涉及的分子变化。使用Illumina Hi-seq测序技术对对照组（CF）和感染鱼组（IF）的RNA进行测序。对于对照组，产生了28,952,608条clean reads，并进行了从头组装以产生60,753个重叠群。对于真菌感染组，获得了25,470,920条clean reads，并组装产生了58,654个重叠群。差异基因表达分析显示，共有146个基因上调，486个基因下调。大多数差异表达基因参与先天免疫机制，如病原体识别、信号传导和抗菌机制。有趣的是，少数适应性免疫基因，尤其是免疫球蛋白在真菌感染期间也显著上调。此外，结果通过qRT-PCR分析得到验证。这些结果表明，条纹鳢在真菌感染期间，多种免疫基因参与了先天和适应性免疫机制，这为乌鳢针对入侵丝囊霉菌的免疫机制提供了新的见解。
