使用 Cy3 染料对 DNA 进行荧光“光点击”标记。
Fluorogenic "photoclick" labelling of DNA using a Cy3 dye.
机构信息
Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany.
出版信息
Org Biomol Chem. 2018 Nov 7;16(41):7579-7582. doi: 10.1039/c8ob02068j. Epub 2018 Oct 11.
Two 2'-deoxyuridines as new building blocks for automated DNA synthesis carry a small aryltetrazole as "photoclickable" group at their 5-positions. The postsynthetic "photoclick" labeling of such presynthesized DNA using a maleimide-modified Cy3 dye shows an up to 17-fold fluorogenicity due to an energy transfer between the pyrazoline moiety and the Cy3 fluorophore in the DNA products. This concept is also applicable to other maleimide-modified dyes.
两个 2'-脱氧尿嘧啶核苷作为新的结构单元，在其 5-位带有一个小的芳基四唑作为“光点击”基团。使用马来酰亚胺修饰的 Cy3 染料对这种预合成的 DNA 进行的“光点击”后标记，由于吡唑啉部分和 DNA 产物中 Cy3 荧光团之间的能量转移，荧光性提高了 17 倍。该概念也适用于其他马来酰亚胺修饰的染料。
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