利用带有半羧基翁盐的荧光“点击”反应对寡核苷酸进行标记。
Oligonucleotide labelling using a fluorogenic "click" reaction with a hemicarboxonium salt.
机构信息
Modified Nucleic Acids, Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique (UMR 5068) Université de Toulouse (Université Paul Sabatier), Cedex 9, 31062 Toulouse, France.
出版信息
Molecules. 2013 Oct 17;18(10):12966-76. doi: 10.3390/molecules181012966.
Abstract
Two fluorescent streptocyanine labelled oligonucleotides have been synthesized by a simple "click" reaction between a non-fluorescent hemicarboxonium salt and aminoalkyl functionalized thymidines within the oligonucleotide and their spectrophotometric properties have been studied.
摘要
两种荧光streptocyanine 标记的寡核苷酸已通过非荧光半羧基铵盐与寡核苷酸中氨基烷基化胸腺嘧啶之间的简单“点击”反应合成，并研究了它们的分光光度性质。
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