用于测定抗脂生成化合物的灵敏、快速且特异的生物测定法。
Sensitive, rapid, and specific bioassay for the determination of antilipogenic compounds.
作者信息
Ulitzur S, Goldberg I
出版信息
Antimicrob Agents Chemother. 1977 Sep;12(3):308-13. doi: 10.1128/AAC.12.3.308.
Abstract
A sensitive and rapid bioassay for the determination of the antilipogenic compounds cerulenin and CM-55 is described. The bioassay is based on the inhibitory effect of cerulenin and CM-55 on the in vivo luminescence of an aldehyde-requiring mutant of the marine bacterium Beneckea harveyi. A total quantity as low as 0.1 mug of cerulenin can be determined within 15 min with an error of +/-2%. The bioassay, as presented, is specific for compounds that are known to inhibit fatty acid biosynthesis and, as such, it might be used as a general screening method for the detection of antilipogenic compounds.
摘要
本文描述了一种灵敏且快速的生物测定法，用于测定抗脂生成化合物浅蓝菌素和CM - 55。该生物测定法基于浅蓝菌素和CM - 55对海洋细菌哈维贝内克氏菌（Beneckea harveyi）的一个需醛突变体的体内发光的抑制作用。在15分钟内可测定低至0.1微克的浅蓝菌素总量，误差为±2%。所介绍的生物测定法对已知抑制脂肪酸生物合成的化合物具有特异性，因此，它可用作检测抗脂生成化合物的通用筛选方法。
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引用本文的文献
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Inhibition of Vibrio harveyi bioluminescence by cerulenin: in vivo evidence for covalent modification of the reductase enzyme involved in aldehyde synthesis.浅蓝菌素对哈维氏弧菌生物发光的抑制作用：醛合成相关还原酶共价修饰的体内证据
J Bacteriol. 1989 Jul;171(7):3866-71. doi: 10.1128/jb.171.7.3866-3871.1989.
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Elongation of exogenous fatty acids by the bioluminescent bacterium Vibrio harveyi.哈维氏弧菌对外源脂肪酸的延长作用。
J Bacteriol. 1989 Jan;171(1):59-64. doi: 10.1128/jb.171.1.59-64.1989.
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Growth, luminescence, respiration, and the ATP pool during autoinduction in Beneckea harveyi.哈维氏贝内克氏菌自诱导过程中的生长、发光、呼吸及ATP库
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本文引用的文献
1
The inhibition of bacterial luciferase by mixed function oxidase inhibitors.混合功能氧化酶抑制剂对细菌荧光素酶的抑制作用。
J Biol Chem. 1972 Feb 10;247(3):888-94.
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Revised structure of cerulenin.
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Inhibition of fatty acid synthetases by the antibiotic cerulenin.抗生素浅蓝菌素对脂肪酸合成酶的抑制作用。
Biochem Biophys Res Commun. 1972 Aug 7;48(3):649-56. doi: 10.1016/0006-291x(72)90397-x.
4
Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast.抗脂肪生成抗生素浅蓝菌素在酵母中对甾醇合成的抑制靶点。
Biochem Biophys Res Commun. 1974 Apr 23;57(4):1119-24. doi: 10.1016/0006-291x(74)90812-2.
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Inhibition of fatty acid synthesis by the antibiotic cerulenin. Specific inactivation of beta-ketoacyl-acyl carrier protein synthetase.抗生素浅蓝菌素对脂肪酸合成的抑制作用。β-酮酰-酰基载体蛋白合成酶的特异性失活。
Biochim Biophys Acta. 1973 Nov 29;326(2):155-6. doi: 10.1016/0005-2760(73)90241-5.
6
The action mechanism of cerulenin. I. Effect of cerulenin on sterol and fatty acid biosynthesis in yeast.浅蓝菌素的作用机制。I. 浅蓝菌素对酵母中甾醇和脂肪酸生物合成的影响。
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7
Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer.利用细菌荧光素酶和液体闪烁光谱仪测定亚皮摩尔水平的NADH和FMN。
Anal Biochem. 1971 Feb;39(2):441-53. doi: 10.1016/0003-2697(71)90434-9.
8
Mechanism of action of CM-55, a synthetic analogue of the antilipogenic antibiotic cerulenin.抗脂肪生成抗生素浅蓝菌素的合成类似物CM-55的作用机制。
Antimicrob Agents Chemother. 1974 Oct;6(4):387-92. doi: 10.1128/AAC.6.4.387.
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The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis.抗生素浅蓝菌素，作为一种脂肪酸合成抑制剂，是生物化学领域的一种新型工具。
Bacteriol Rev. 1976 Sep;40(3):681-97. doi: 10.1128/br.40.3.681-697.1976.
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