Ulitzur S, Goldberg I
Antimicrob Agents Chemother. 1977 Sep;12(3):308-13. doi: 10.1128/AAC.12.3.308.
A sensitive and rapid bioassay for the determination of the antilipogenic compounds cerulenin and CM-55 is described. The bioassay is based on the inhibitory effect of cerulenin and CM-55 on the in vivo luminescence of an aldehyde-requiring mutant of the marine bacterium Beneckea harveyi. A total quantity as low as 0.1 mug of cerulenin can be determined within 15 min with an error of +/-2%. The bioassay, as presented, is specific for compounds that are known to inhibit fatty acid biosynthesis and, as such, it might be used as a general screening method for the detection of antilipogenic compounds.
本文描述了一种灵敏且快速的生物测定法，用于测定抗脂生成化合物浅蓝菌素和CM - 55。该生物测定法基于浅蓝菌素和CM - 55对海洋细菌哈维贝内克氏菌（Beneckea harveyi）的一个需醛突变体的体内发光的抑制作用。在15分钟内可测定低至0.1微克的浅蓝菌素总量，误差为±2%。所介绍的生物测定法对已知抑制脂肪酸生物合成的化合物具有特异性，因此，它可用作检测抗脂生成化合物的通用筛选方法。
